Deciphering RNA splicing logic with interpretable machine learning.

Liao SE, Sudarshan M, Regev O
Proc Natl Acad Sci U S A. 2023 Oct 10;120(41):e2221165120. doi: 10.1073/pnas.2221165120. Epub 2023 Oct 5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
204143	pRegev_V1	Single reporter randomly selected from library for biochemical validation
204144	pRegev_V2	Single reporter randomly selected from splicing library for biochemical validation.
204145	pRegev_V3	Single reporter randomly selected from splicing library for biochemical validation
204150	pRegev_S1	Single reporter with secondary structure from splicing library.
204160	pRegev_S1.46G>U	pRegev_S1 with single mutation that disrupts secondary structure.
204161	pRegev_S1.34C>A_46G>U	pRegev_S1 with double mutations that restore secondary structure.
204162	pRegev_S2	Single reporter with secondary structure from splicing library.
204163	pRegev_S2.39U>G	pRegev_S2 with single mutation that disrupts secondary structure.
204165	pRegev_S2.39U>G.51A>C	pRegev_S2 with double mutations that restore secondary structure.
204170	pRegev_S4	Single reporter with secondary structure from splicing library.
204171	pRegev_S4.39U>G	pRegev_S4 with single mutation that disrupts secondary structure.
204172	pRegev_S4.51A>C	pRegev_S4 with single mutation that disrupts secondary structure.
204173	pRegev_S4.39U>G.51A>C	pRegev_S4 with double mutations that restore secondary structure.
204174	pRegev_S5	Single reporter with secondary structure from splicing library.
204175	pRegev_S5.49C>A	pRegev_S5 with single mutation that disrupts secondary structure.
204177	pRegev_S5.49C>A.59G>U	pRegev_S5 with double mutations that restore secondary structure.
204178	pRegev_D1	Single reporter with long guanine depleted (G-poor) region from splicing library.
204179	pRegev_D1.61C>G	pRegev_D1 with single C>G mutation.
204180	pRegev_D2	Single reporter with long guanine depleted (G-poor) region from splicing library.
204181	pRegev_D2.30C>G	pRegev_D2 with single C>G mutation.
204182	pRegev_D3	Single reporter with long guanine depleted (G-poor) region from splicing library.
204183	pRegev_D3.48C>G	pRegev_D3 with single C>G mutation.
204184	pRegev_D4	Single reporter with long guanine depleted (G-poor) region from splicing library.
204185	pRegev_D4.34C>G	pRegev_D4 with single C>G mutation.
204186	pRegev_ExonSkip_LandingPad	Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.
204187	pRegev_ExonSkip_Backbone	Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).