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Comparative Evaluation of Synthetic Cytokines for Enhancing Production and Performance of NK92 Cell-Based Therapies.

Deol S, Donahue PS, Mitrut RE, Hammitt-Kess IJ, Ahn J, Zhang B, Leonard JN
GEN Biotechnol. 2023 Jun 1;2(3):228-246. doi: 10.1089/genbio.2023.0024. Epub 2023 Jun 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248029pGIPZ EF1a IRES miRFP720-P2A-BlastBackbone that constitutively expresses miRFP720 and a blasticidin resistance gene in human cells
248030pGIPZ EF1a IRES mNeonGreen-P2A-PuroBackbone that constitutively expresses mNeonGreen and a puromycin resistance gene in human cells
248031pGIPZ EF1a myc tagged mbIL2 IRES miRFP720-P2A-BlastConstitutively expresses myc tagged membrane bound IL2, miRFP720 and a blasticidin resistance gene in human cells
248032pGIPZ EF1a myc tagged mbIL15 IRES miRFP720-P2A-BlastConstitutively expresses myc tagged membrane bound IL15, miRFP720 and a blasticidin resistance gene in human cells
248033pGIPZ EF1a spnull-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-PuroConstitutively expresses 3xFLAG tagged Neoleukin 2/15, mNeonGreen and a puromycin resistance gene in human cells
248034pGIPZ EF1a spIgE-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro Constitutively expresses 3xFLAG tagged Neoleukin 2/15 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells
248035pGIPZ EF1a ACCx2 uORF eIL12 IRES mNeonGreen-P2A-PuroConstitutively expresses an engineered version of IL12, mNeonGreen and a puromycin resistance gene in human cells
248036pGIPZ EF1a eIL15 IRES mNeonGreen-P2A-PuroConstitutively expresses an engineered version of IL15, mNeonGreen and a puromycin resistance gene in human cells
248037pGIPZ EF1a spnull-3xFLAG-DR18 IRES mNeonGreen-P2A-PuroConstitutively expresses 3xFLAG tagged DR18, mNeonGreen and a puromycin resistance gene in human cells
248038pGIPZ EF1a spIgE-3xFLAG-DR18 IRES mNeonGreen-P2A-PuroConstitutively expresses 3xFLAG tagged DR18 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells

Antibodies from Article