Rapid and Quantitative Functional Interrogation of Human Enhancer Variant Activity in Live Mice
Hollingsworth EW, Liu TA, Jacinto SH, Chen CX, Alcantara JA, Kvon EZ
bioRxiv 2023.12.10.570890 Article
Hollingsworth EW, Liu TA, Jacinto SH, Chen CX, Alcantara JA, Kvon EZ
bioRxiv 2023.12.10.570890 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211940 | PCR4-Hsp68::mCherry-H11 | dual-enSERT-1 mCherry vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter) |
| 211941 | PCR4-Hsp68::eGFP-H11 | dual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter) |
| 211942 | PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11 | dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator) |
| 216394 | PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11 | dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator) |