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An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes.

Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 10.1016/j.neuron.2025.05.002. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
213814pAAV_BiSSTe10_dTomato_nlsdTomatoAAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_dTomato_nlsdTomato
213815pAAV_BiSSTe10_ChR2_mCherryAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry
213816pAAV_BiSSTe10_C1V1_eYFPAAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_C1V1_eYFP
213817pAAV_BiSSTe10_Gq_dTomatoAAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_Gq_dTomato
213818pAAV_BiSSTe10_GCaMP6fAAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_GCaMP6f
213829pAAV_BiCHATe27_dTomato_nlsdTomatoAAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomato
213830pAAV_BiCHATe27_ChR2_mCherryAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_ChR2_mCherry
213831pAAV_BiCHATe27_C1V1_eYFPAAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_C1V1_eYFP
213832pAAV_BiCHATe27_Gq_dTomatoAAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato
213833pAAV_BiCHATe27_GCaMP6fAAV construct expressing GCaMP6f driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_GCaMP6f
213855pAAV_BiVIPe4_dTomato_nlsdTomatoAAV construct expressing dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_dTomato_nlsdTomato
213856pAAV_BiVIPe4_ChR2_mCherryAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry
213858pAAV_BiVIPe4_Gq_dTomatoAAV construct expressing HA-hM3Dq-dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_Gq_dTomato
213859pAAV_BiVIPe4_GCaMP6fAAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6f
213915pAAV_BiLAMP5e3_ChR2_mCherryAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_ChR2_mCherry
213916pAAV_BiLAMP5e3_Gq_dTomatoAAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomato
213917pAAV_BiLAMP5e3_GCaMP6fAAV construct expressing GCaMP6f driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_GCaMP6f
213918pAAV_BiLAMP5e3_jGCaMP8mAAV construct expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_jGCaMP8m
213936pAAV_BiPVe4_dTomato_nlsdTomatoAAV construct expressing dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_dTomato_nlsdTomato
213937pAAV_BiPVe4_ChR2_mCherryAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry
213938pAAV_BiPVe4_Gq_dTomatoAAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato
213939pAAV_BiPVe4_GCaMP6fAAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6f
213940pAAV_BiPVe3_dTomato_nlsdTomatoAAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WP0003_dTomato_nlsdTomato
213941pAAV_BiPVe3_ChR2_mCherryAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherry
213942pAAV_BiPVe3_Gq_dTomatoAAV construct expressing HA-hM3Dq-dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_Gq_dTomato
213943pAAV_BiPVe3_GCaMP6fAAV construct expressing GCaMP6f driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_GCaMP6f
213944pAAV_BiSSTe4_dTomato_nlsdTomatoAAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: AAV_WDS0004_dTomato_nlsdTomato
213945pAAV_BiSSTe4_ChR2_mCherryAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_ChR2_mCherry
213946pAAV_BiSSTe4_Gq_dTomatoAAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato
213947pAAV_BiSSTe4_GCaMP6fAAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f
218792pAAV_BiLAMP5e3_dTomato_nlsdTomatoAAV construct with dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato
221463pAAV_BiPVe4_eGFPAAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

Antibodies from Article