Directed Evolution of a Bacterial Leucyl tRNA in Mammalian Cells for Enhanced Noncanonical Amino Acid Mutagenesis.
Huang RL, Jewel D, Kelemen RE, Pham Q, Yared TJ, Wang S, Singha Roy SJ, Huang Z, Levinson SD, Sundaresh B, Miranda SE, van Opijnen T, Chatterjee A
ACS Synth Biol. 2024 Jul 19;13(7):2141-2149. doi: 10.1021/acssynbio.4c00196. Epub 2024 Jun 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217365 | pIDTSmart-CMV-PLRS1 | Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific" |
| 217366 | pAAV-ITR-CMV-mCherry-1xU6-LtR-wt(TAG) | Expresses E. coli leucine tRNA and a wild-type mCherry reporter; can be packaged into AAV |
| 217367 | pAAV-ITR-CMV-mCherry-1xU6-LeuIGI1 | Expresses E. coli leucine tRNA variant "LeuIGI1" and a wild-type mCherry reporter; can be packaged into AAV |
| 217368 | pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2 | Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV |