Evidence for the nuclear import of histones H3.1 and H4 as monomers.
Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ
EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub 2018 Sep 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217962 | pCL1032 | Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES. |
| 217963 | pCL1271 | Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1. |
| 252410 | pCL0639 | NASP tagged on the N-terminus with mCherry. |
| 252411 | pCL0918 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252412 | pCL0919 | Auto-Inhibited TVMV protease for the RAPID-release approach |
| 252413 | pCL0941 | Auto-Inhibited TVMV protease for the RAPID-release approach |
| 252414 | pCL0943 | Auto-Inhibited TVMV protease for the RAPID-release approach |
| 252415 | pCL0945 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252416 | pCL0946 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252417 | pCL0948 | Uninhibited TVMV protease for the RAPID-release approach |
| 252418 | pCL0950 | KPNB1 tagged on the N-terminus with mCherry. |
| 252419 | pCL0952 | IPO11 tagged on the N-terminus with mCherry. |
| 252420 | pCL0956 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252421 | pCL0960 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252422 | pCL0965 | IPO4 tagged on the N-terminus with mCherry. |
| 252424 | pCL0978 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252425 | pCL0979 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252427 | pCL0981 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252428 | pCL1002 | mCherry-tagged NASP with nuclear localisation sequence (NLS) deleted |
| 252429 | pCL1008 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252430 | pCL1009 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252431 | pCL1010 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252432 | pCL1011 | RbAp46 tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic. |
| 252433 | pCL1013 | HAT1 tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic. |
| 252434 | pCL1023 | ASF1A tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic. |
| 252436 | pCL1046 | Mitochondria-tethered construct for the RAPID-Release approach |
| 252437 | pCL1052 | ASF1b tagged on the N-terminus with TEV-cleavable eGFP and selection with puromycin |
| 252438 | pCL1054 | HAT1 tagged on the N-terminus with TEV-cleavable eGFP and selection with puromycin |