Functional implications of hexameric dynamics in SARS-CoV-2 Nsp15.
Sonowal M, Ketawala G, Nagaratnam N, Logeswaran D, Basu S, de Sanctis D, Orlans J, Rose SL, Jernigan RJ, Hu H, Aguilar JDM, Ranaweera ME, Zacks MA, Chen JJ, Hansen DT, Schrag LG, Fromme R, Botha S, Fromme P
Protein Sci. 2025 Jun;34(6):e70115. doi: 10.1002/pro.70115.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218803 | pHis-TEV-Nsp15-E266Q | E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag |
| 219524 | pHis-TEV-Nsp15-V66L | E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag |
| 219525 | pHis-TEV-Nsp15-A81V | E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag |
| 219526 | pHis-TEV-Nsp15-D183N | E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag |
| 219529 | pHis-TEV-Nsp15-H234A | E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag |