PILS-Nir1 is a sensitive phosphatidic acid biosensor that reveals mechanisms of lipid production.
Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV
J Cell Biol. 2025 Nov 3;224(11):e202405174. doi: 10.1083/jcb.202405174. Epub 2025 Sep 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220081 | pNES-EGFP-C1-flex-PABD-Spo20 | Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export |
| 220082 | pNESx2-EGFP-C1-PABDx2-Spo20 | PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export |
| 220083 | pNeonGreen-C1-Nir1-LNS2 | High affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1) |
| 220084 | pmCherry-C1-Nir1-LNS2 | high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1) |
| 220085 | pmiRFP670-C1-Nir1-LNS2 | high affinity PA biosensor with far-red fluorophore |
| 220086 | pNeonGreen-C1-Nir2-LNS2 | C-terminal PA binding domain of Nir2 |
| 220087 | pmEGFP-C1-Nir3-LNS2 | C-terminal PA binding domain of Nir3 |
| 220088 | pNeonGreen-N1-Nir1-613-630 | N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA |
| 220089 | pNeonGreen-C1-Nir1-631-894 | Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA |
| 220090 | pTagBFP-C1-FKBP-PIP5K1C-HD-EE | Recruitable PIP5K to make PIP2 |