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PILS-Nir1 is a sensitive phosphatidic acid biosensor that reveals mechanisms of lipid production.

Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV
J Cell Biol. 2025 Nov 3;224(11):e202405174. doi: 10.1083/jcb.202405174. Epub 2025 Sep 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
220081pNES-EGFP-C1-flex-PABD-Spo20Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export
220082pNESx2-EGFP-C1-PABDx2-Spo20PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export
220083pNeonGreen-C1-Nir1-LNS2High affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)
220084pmCherry-C1-Nir1-LNS2high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)
220085pmiRFP670-C1-Nir1-LNS2high affinity PA biosensor with far-red fluorophore
220086pNeonGreen-C1-Nir2-LNS2C-terminal PA binding domain of Nir2
220087pmEGFP-C1-Nir3-LNS2C-terminal PA binding domain of Nir3
220088pNeonGreen-N1-Nir1-613-630N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA
220089pNeonGreen-C1-Nir1-631-894Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA
220090pTagBFP-C1-FKBP-PIP5K1C-HD-EERecruitable PIP5K to make PIP2

Antibodies from Article