Open-ended molecular recording of sequential cellular events into DNA.

Loveless TB, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
Nat Chem Biol. 2025 Apr;21(4):512-521. doi: 10.1038/s41589-024-01764-5. Epub 2024 Nov 14. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
226661	pTBL2142 MTK3-sfGFP	MTK3 part containing the coding sequence of sfGFP
226662	pTBL3445 MTK3-mTagBFP2	MTK3 part containing the coding sequence of mTagBFP2
226663	pTBL2670 MTK3-PEmax	MTK3 part containing the coding sequence of PEmax
226664	pTBL3478 PB-PEmax-mTagBFP2	PiggyBac cargo vector encoding PEmax and mTagBFP2
226665	pTBL3346 PB-nicking guide-blast	PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin
226666	pTBL3241 PB-AtoBpegRNA-mCherry	PiggyBac cargo vector encoding A to B pegRNA marked with mCherry
226667	pTBL3401 BtoApegRNA-sfGFP	PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP
226668	pTBL3331 MTK234-TetO-AtoBpegRNA	MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA
226669	pTBL3428 MTK234-LacO-BtoApegRNA	MTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNA
226670	pTBL2620 MTK3-TetR	MTK3 part containing the coding sequence of TetR
226671	pTBL2621 MTK3-LacI	MTK3 part containing the coding sequence of LacI
226672	pTBL3481 MTK3-mCherry	MTK3 part containing the coding sequence of mCherry.
226673	pTBL2368 MTK PB puro backbone	Empty MTK PB cargo backbone marked with puromycin resistance.
226674	pTBL3342 PB-PEmax-puro	PiggyBac cargo vector encoding PEmax and puromycin resistance.
226675	pTBL2367 MTK PB empty backbone with no marker	Empty MTK PB cargo backbone with no marker.
226676	pTBL3328 PB-sfGFP-WPRE-target	PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.