Characterizing and controlling CRISPR repair outcomes in nondividing human cells.
Ramadoss GN, Namaganda SJ, Kumar MM, Hamilton JR, Sharma R, Chow KG, Workley LA, Macklin BL, Sun M, Ha AS, Liu JC, Fellmann C, Watry HL, Dierks PH, Bose RS, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Shy BR, Nussenzweig A, Adamson B, Murthy N, Doudna JA, Kampmann M, Conklin BR
Nat Commun. 2025 Nov 17;16(1):9883. doi: 10.1038/s41467-025-66058-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225959 | pCF140_Gag-SpyCas9-NLS | CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production. |
| 225960 | pCF142_U6-sgRNA | U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs. |
| 225961 | pCF153_Gag-Pol | CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production. |
| 225962 | pCF159_BRL | CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production. |
| 225963 | pCF160_VSV-G | CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production. |