Functional spectrum of USP7 pathogenic variants in Hao-Fountain syndrome: Insights into the enzyme's activity, stability, and allosteric modulation.
Korchak EJ, Sharafi M, Jaen Maisonet I, Salazar-Chaparro A, Semenova IV, Khan H, O'Neil AL, Caro P, Schaaf CP, Buhrlage SJ, Bezsonova I
Proc Natl Acad Sci U S A. 2025 Sep 30;122(39):e2510252122. doi: 10.1073/pnas.2510252122. Epub 2025 Sep 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227201 | WT USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227202 | USP7 N418S catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227203 | USP7 G392D catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227204 | G392A USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227205 | Y331H USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227206 | M225I USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227207 | L373F USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227208 | P273H USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227209 | V485G USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227210 | K420E USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227211 | E345K USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227212 | L469R USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227213 | L299A USP7 catalytic | Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli |
| 227214 | WT FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227215 | N418S FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227216 | G392D FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227217 | G392A FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227218 | Y331H FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227219 | M225I FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227220 | L373F FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227221 | P273H FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227222 | V485G FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227223 | K420E FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227224 | E345K FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 227225 | L469R FL USP7 | Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli |
| 229001 | E547* FL USP7 | E547-mutant of human USP7 |