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Long-range regulation of transcription scales with genomic distance in a gene-specific manner.

Jensen CL, Chen LF, Swigut T, Crocker OJ, Yao D, Bassik MC, Ferrell JE Jr, Boettiger AN, Wysocka J
Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
227444pk335-CARGO-6mer-26kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227445pk335-CARGO-6mer-24kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227446pk335-CARGO-6mer-20kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227447pk335-CARGO-6mer-11kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227448pk335-CARGO-6mer-7.5kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227449pk335-CARGO-6mer-4.5kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227450pk335-CARGO-6mer-3.3kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227451pk335-CARGO-6mer-2.1kb-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227452pk335-CARGO-6mer-700bp-USP6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227453pk335-CARGO-6mer-PrPro6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227454pk335-CARGO-5mer-PrPro5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227455pk335-CARGO-4mer-PrPro4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227456pk335-CARGO-3mer-PrPro3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227457pk335-CARGO-6mer-58kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227458pk335-CARGO-6mer-55kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227459pk335-CARGO-6mer-42kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227460pk335-CARGO-6mer-40kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227461pk335-CARGO-6mer-36kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227462pk335-CARGO-5mer-36kb-USF5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227463pk335-CARGO-4mer-36kb-USF4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227464pk335-CARGO-3mer-36kb-USF3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227465pk335-CARGO-6mer-33kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227466pk335-CARGO-6mer-29kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227467pk335-CARGO-6mer-26kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227468pk335-CARGO-6mer-25kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227469pk335-CARGO-6mer-18kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227470pk335-CARGO-6mer-14kb-USF-1-66-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227471pk335-CARGO-6mer-14kb-USF-7-126-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227472pk335-CARGO-12mer-14kb-USF-1-1212-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227473pk335-CARGO-6mer-11kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227474pk335-CARGO-6mer-7.1kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227475pk335-CARGO-6mer-3.3kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227476pk335-CARGO-6mer-1.9kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227477pk335-CARGO-6mer-2.4kb-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227478pk335-CARGO-6mer-700bp-USF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227479pk335-CARGO-6mer-Fgf5Pro6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227480pk335-CARGO-5mer-Fgf5Pro5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227481pk335-CARGO-4mer-Fgf5Pro4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227482pk335-CARGO-6mer-26kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227483pk335-CARGO-6mer-30kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227484pk335-CARGO-5mer-30kb-DSF5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227485pk335-CARGO-4mer-30kb-DSF4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227486pk335-CARGO-3mer-30kb-DSF3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227487pk335-CARGO-6mer-35kb-DSF-1-66-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227488pk335-CARGO-6mer-35kb-DSF-6-116-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227489pk335-CARGO-11mer-35kb-DSF-1-1111-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227490pk335-CARGO-5mer-35kb-DSF5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227491pk335-CARGO-4mer-35kb-DSF4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227492pk335-CARGO-3mer-35kb-DSF3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227494pk335-CARGO-6mer-44kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227495pk335-CARGO-6mer-48kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227496pk335-CARGO-6mer-51kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227497pk335-CARGO-6mer-66kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227498pk335-CARGO-6mer-70kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227499pk335-CARGO-6mer-73kb-DSF6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
227500pk335-NT-1mer-gRNAnon-targeting control guide
227501pCLJ-P2F-Frt-PGK-Puro-FrtInserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassette
227502pCLJ-F2P-LoxP-PGK-PuroR-LoxPInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette
227503pCLJ-F2P-LoxP-PGK-NeoR-LoxPInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette
227504pCLJ-Fgf5-t2A-eGFPInserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette
227505pk335-BFPBackbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence
227506pB-tre-dCas9-VPRpiggyBac integration and dox-inducible expression of VPR-dCas9

Antibodies from Article