Long-range regulation of transcription scales with genomic distance in a gene-specific manner.
Jensen CL, Chen LF, Swigut T, Crocker OJ, Yao D, Bassik MC, Ferrell JE Jr, Boettiger AN, Wysocka J
Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227444 | pk335-CARGO-6mer-26kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227445 | pk335-CARGO-6mer-24kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227446 | pk335-CARGO-6mer-20kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227447 | pk335-CARGO-6mer-11kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227448 | pk335-CARGO-6mer-7.5kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227449 | pk335-CARGO-6mer-4.5kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227450 | pk335-CARGO-6mer-3.3kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227451 | pk335-CARGO-6mer-2.1kb-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227452 | pk335-CARGO-6mer-700bp-USP | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227453 | pk335-CARGO-6mer-PrPro | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227454 | pk335-CARGO-5mer-PrPro | 5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227455 | pk335-CARGO-4mer-PrPro | 4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227456 | pk335-CARGO-3mer-PrPro | 3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227457 | pk335-CARGO-6mer-58kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227458 | pk335-CARGO-6mer-55kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227459 | pk335-CARGO-6mer-42kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227460 | pk335-CARGO-6mer-40kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227461 | pk335-CARGO-6mer-36kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227462 | pk335-CARGO-5mer-36kb-USF | 5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227463 | pk335-CARGO-4mer-36kb-USF | 4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227464 | pk335-CARGO-3mer-36kb-USF | 3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227465 | pk335-CARGO-6mer-33kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227466 | pk335-CARGO-6mer-29kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227467 | pk335-CARGO-6mer-26kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227468 | pk335-CARGO-6mer-25kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227469 | pk335-CARGO-6mer-18kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227470 | pk335-CARGO-6mer-14kb-USF-1-6 | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227471 | pk335-CARGO-6mer-14kb-USF-7-12 | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227472 | pk335-CARGO-12mer-14kb-USF-1-12 | 12-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227473 | pk335-CARGO-6mer-11kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227474 | pk335-CARGO-6mer-7.1kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227475 | pk335-CARGO-6mer-3.3kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227476 | pk335-CARGO-6mer-1.9kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227477 | pk335-CARGO-6mer-2.4kb-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227478 | pk335-CARGO-6mer-700bp-USF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227479 | pk335-CARGO-6mer-Fgf5Pro | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227480 | pk335-CARGO-5mer-Fgf5Pro | 5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227481 | pk335-CARGO-4mer-Fgf5Pro | 4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227482 | pk335-CARGO-6mer-26kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227483 | pk335-CARGO-6mer-30kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227484 | pk335-CARGO-5mer-30kb-DSF | 5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227485 | pk335-CARGO-4mer-30kb-DSF | 4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227486 | pk335-CARGO-3mer-30kb-DSF | 3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227487 | pk335-CARGO-6mer-35kb-DSF-1-6 | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227488 | pk335-CARGO-6mer-35kb-DSF-6-11 | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227489 | pk335-CARGO-11mer-35kb-DSF-1-11 | 11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227490 | pk335-CARGO-5mer-35kb-DSF | 5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227491 | pk335-CARGO-4mer-35kb-DSF | 4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227492 | pk335-CARGO-3mer-35kb-DSF | 3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227494 | pk335-CARGO-6mer-44kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227495 | pk335-CARGO-6mer-48kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227496 | pk335-CARGO-6mer-51kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227497 | pk335-CARGO-6mer-66kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227498 | pk335-CARGO-6mer-70kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227499 | pk335-CARGO-6mer-73kb-DSF | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus |
| 227500 | pk335-NT-1mer-gRNA | non-targeting control guide |
| 227501 | pCLJ-P2F-Frt-PGK-Puro-Frt | Inserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassette |
| 227502 | pCLJ-F2P-LoxP-PGK-PuroR-LoxP | Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette |
| 227503 | pCLJ-F2P-LoxP-PGK-NeoR-LoxP | Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette |
| 227504 | pCLJ-Fgf5-t2A-eGFP | Inserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette |
| 227505 | pk335-BFP | Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence |
| 227506 | pB-tre-dCas9-VPR | piggyBac integration and dox-inducible expression of VPR-dCas9 |