Genetically encoded reporters of actin filament organization in living cells and tissues.
Martins CS, Iv F, Suman SK, Panagiotou TC, Sidor C, Ruso-Lopez M, Plancke CN, Omi S, Pages R, Gomes M, Llewellyn A, Bandi SR, Ramond L, Arbizzani F, Rimoli CV, Schnorrer F, Robin F, Wilde A, LeGoff L, Pedelacq JD, Jegou A, Cabantous S, Rincon SA, Chandre C, Brasselet S, Mavrakis M
Cell. 2025 Apr 1:S0092-8674(25)00276-4. doi: 10.1016/j.cell.2025.03.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231548 | pCMVtrunc msfGFPΔC10-beta-actin | Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy |
| 231549 | pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin | Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization |
| 231550 | pCMVtrunc Affimer6-msfGFPΔN12 | Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy |
| 231551 | pCMVtrunc Affimer6-sfCherry2ΔN12 | Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy |
| 231552 | pCMVtrunc F-tractinN9-39-msfGFPΔN7 | Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy |
| 231553 | iGFP-beta-actin | Mammalian expression of human beta actin fused intramolecularly to monomeric superfolder GFP |
| 231554 | iGFP-gamma-actin | Mammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFP |
| 231555 | pCMVtrunc LifeactΔC4-msfGFPΔN7 | Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy |
| 231556 | pCMVtrunc msfGFPΔC9-LifeactΔN2 | Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy |
| 231557 | pCMVtrunc sfCherry2ΔC4-Lifeact | Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy |
| 231558 | pCMVtrunc msfGFPΔC10-Utr28-222 | Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy |