Proliferation history and transcription factor levels drive direct conversion to motor neurons.
Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, Galloway KE
Cell Syst. 2025 Apr 16;16(4):101205. doi: 10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233148 | pMXs-mNgn2 | Retroviral expression of Ngn2 |
| 233149 | pMXs-Isl1 | Retroviral expression of Isl1 |
| 233150 | pMXs-mLhx3 | Retroviral expression of Lhx3 |
| 233151 | pMXs-mBrn2 | Retroviral expression of Brn2 |
| 233152 | pMXs-mAscl1 | Retroviral expression of Ascl1 |
| 233153 | pMXs-hMyt1l | Retroviral expression of Myt1l |
| 233154 | pMXs-NIL | Retroviral expression of Ngn2, Isl1, and Lhx3 for motor neuron reprogramming |
| 233155 | pMXs-mRuby2 | Retroviral expression of fluorescent mRuby2 reporter |
| 233156 | pMXs-mBrn2-P2A-mRuby2 | Retroviral expression of fluorescent mRuby2 reporter |
| 233157 | pENTR-mNgn2x3HA-P2A-mRuby2 | Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter |
| 233158 | pENTR-Isl1-P2A-mRuby2 | Retroviral expression of Isl1 with mRuby2 fluorescent reporter |
| 233159 | pENTR-mLhx3-P2A-mRuby2 | Retroviral expression of mLhx3 with mRuby2 fluorescent reporter |
| 233160 | pMXs-Isl1-T2A-mLhx3 | Retroviral expression of Isl1 and Lhx3 for motor neuron specification |
| 233161 | pMXs-mNgn2x3HA-T2A-Isl1 | Retroviral expression of mNgn2x3HA and Isl1 |
| 233162 | pMXs-mNgn2x3HA-T2A-mLhx3 | Retroviral expression of mNgn2x3HA and Lhx3 |
| 233163 | pMXs-mNgn2x3HA-P2A-mRuby2 | Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter |
| 233164 | pMXs-uORFwx1-mNgn2x3HA-P2A-mRuby2 | Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame |
| 233165 | pMXs-uORFx1-mNgn2x3HA-P2A-mRuby2 | Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame |
| 233166 | pMXs-uORFx2-mNgn2x3HA-P2A-mRuby2 | Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame |
| 233167 | pMXs-uORFx3-mNgn2x3HA-P2A-mRuby2 | Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame |
| 233168 | pMXs-uORFx6-mNgn2x3HA-P2A-mRuby2 | Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame |
| 233169 | pMXs-Isl1-P2A-mRuby2 | Retroviral expression of Isl1 with mRuby2 fluorescent reporter |
| 233170 | pMXs-uORFwx1-Isl1-P2A-mRuby2 | Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233171 | pMXs-uORFx1-Isl1-P2A-mRuby2 | Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233172 | pMXs-uORFx2-Isl1-P2A-mRuby2 | Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233173 | pMXs-uORFx3-Isl1-P2A-mRuby2 | Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233174 | pMXs-uORFx6-Isl1-P2A-mRuby2 | Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233175 | pMXs-mLhx3-P2A-mRuby2 | Retroviral expression of mLhx3 with mRuby2 fluorescent reporter |
| 233176 | pMXs-uORFwx1-mLhx3-P2A-mRuby2 | Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233177 | pMXs-uORFx1-mLhx3-P2A-mRuby2 | Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233178 | pMXs-uORFx2-mLhx3-P2A-mRuby2 | Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233179 | pMXs-uORFx3-mLhx3-P2A-mRuby2 | Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233180 | pMXs-uORFx6-mLhx3-P2A-mRuby2 | Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame |
| 233181 | pMXs-HRAS(GV12) | Retroviral expression of HRAS(G12V) |
| 233182 | pMXs-myrAkt-P2A-BCL2 | Retroviral expression of myristoylated Akt1 (myr-Akt) and Bcl2 |
| 233183 | pMXs-cMyc | Retroviral expression of c-Myc |
| 233184 | pMXs-hNeuroD1 | Retroviral expression of NeuroD1 |