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eIF4A1 enhances LARP1-mediated translational repression during mTORC1 inhibition.

Shichino Y, Yamaguchi T, Kashiwagi K, Mito M, Takahashi M, Ito T, Ingolia NT, Kuba K, Iwasaki S
Nat Struct Mol Biol. 2024 Oct;31(10):1557-1566. doi: 10.1038/s41594-024-01321-7. Epub 2024 May 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
233626pcDNA5/FRT/TO-3xFLAG-His6-LARP1Expression of Flag- and His-tagged LARP1
233627pcDNA5/FRT/TO-3xFLAG-His6-LARP1-d327-496Expression of Flag- and His-tagged LARP1delta327-496
233628psiCHECK2-Eef2TOP-RLCP-HCVIRES-FLExpression of bicistronic reporter with TOP motif
233629psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FLExpression of bicistronic reporter with mutated TOP motif

Antibodies from Article