Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cells.
Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, Galloway KE
Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235253 | EFS-mRuby2-bGH | ComMAND base gene regulating mRuby2, EFS-driven |
| 235254 | hPGK-mRuby2-bGH | ComMAND base gene regulating mRuby2, hPGK-driven |
| 235255 | EFS-mGL-bGH | mGreenLantern expression to serve as a transfection marker, EFS-driven |
| 235256 | U6-miR.FF4 | Non-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin) |
| 235257 | EF1a-tagBFP-bGH | tagBFP expression to serve as a "filler" plasmid for transfections |
| 235260 | EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH | ComMAND open-loop circuit regulating mRuby2 with miRE-FF5 |
| 235261 | EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH | ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 |
| 235262 | EF1a-TS.FF6x1-mRuby2(miRE.FF5)-bGH | ComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 2 |
| 235263 | EF1a-TS.FF5x1-mRuby2(miRE.FF5)-bGH | ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 2 |
| 235264 | EF1a-mGL(miRE.FF4)-bGH | microRNA expression for dual-vector iFFL circuit |
| 235265 | EF1a-EGFP-FMRP-bGH | ComMAND EGFP-FMRP base gene |
| 235266 | EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH | ComMAND EGFP-FMRP open-loop circuit |
| 235267 | EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH | ComMAND EGFP-FMRP closed-loop circuit |
| 235268 | pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of ComMAND base gene regulating mRuby2 |
| 235269 | EF1a-mRuby2-bGH | ComMAND base gene regulating mRuby2, EF1a |
| 235270 | EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH | ComMAND open-loop circuit regulating mRuby2, EF1a |
| 235271 | EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH | ComMAND closed-loop circuit regulating mRuby2, EF1a |
| 235272 | EF1a-mRuby2-TS.FF4x1-bGH | 3' microRNA target site expression for dual-vector iFFL circuit (closed-loop) |
| 235273 | EF1a-mRuby2-TS.FF6x1-bGH | 3' microRNA target site expression for dual-vector iFFL circuit (open-loop) |
| 235274 | EF1a-TS.FF4x1-mRuby2-bGH | 3' microRNA target site expression for dual-vector iFFL circuit (closed-loop) |
| 235275 | EF1a-TS.FF6x1-mRuby2-bGH | 3' microRNA target site expression for dual-vector iFFL circuit (open-loop) |
| 235276 | EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH | ComMAND open-loop circuit regulating mRuby2 with miR-FF5 |
| 235277 | EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH | ComMAND closed-loop circuit regulating mRuby2 with miR-FF5 |
| 235278 | EF1a-TS.FF6x1-mRuby2(miR.FF5)-bGH | ComMAND open-loop circuit regulating mRuby2 with miR-FF5 design 2 |
| 235279 | EF1a-TS.FF5x1-mRuby2(miR.FF5)-bGH | ComMAND closed-loop circuit regulating mRuby2 with miR-FF5 design 2 |
| 235280 | EF1a-TS.FF6x1-mRuby2-miR.FF5-bGH | ComMAND open-loop circuit regulating mRuby2 with miR-FF5 design 3 |
| 235281 | EF1a-TS.FF5x1-mRuby2-miR.FF5-bGH | ComMAND closed-loop circuit regulating mRuby2 with miR-FF5 design 3 |
| 235282 | EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH | ComMAND open-loop circuit regulating mRuby2 with miR-FF4 |
| 235283 | EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH | ComMAND closed-loop circuit regulating mRuby2 with miR-FF4 |
| 235284 | EF1a-TS.FF6x1-mRuby2(miR.FF4)-bGH | ComMAND open-loop circuit regulating mRuby2 with miR-FF4 design 2 |
| 235285 | EF1a-TS.FF4x1-mRuby2(miR.FF4)-bGH | ComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 2 |
| 235286 | EF1a-TS.FF6x1-mRuby2-miR.FF4-bGH | ComMAND open-loop circuit regulating mRuby2 with miR-FF4 design 3 |
| 235287 | EF1a-TS.FF4x1-mRuby2-miR.FF4-bGH | ComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 3 |
| 235288 | EF1a-TS.FF6x1-mRuby2(miRE.FF4)-bGH | ComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 2 |
| 235289 | EF1a-TS.FF4x1-mRuby2(miRE.FF4)-bGH | ComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 2 |
| 235290 | EF1a-TS.FF6x1-mRuby2-miRE.FF4-bGH | ComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 3 |
| 235291 | EF1a-TS.FF4x1-mRuby2-miRE.FF4-bGH | ComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 3 |
| 235292 | EF1a-mRuby2(miRE.FF4)-TS.FF6x2-bGH | ComMAND open-loop circuit regulating mRuby2 with 2 copies of microRNA target sites |
| 235293 | EF1a-mRuby2(miRE.FF4)-TS.FF6x4-bGH | ComMAND open-loop circuit regulating mRuby2 with 4 copies of microRNA target sites |
| 235294 | EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH | ComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sites |
| 235296 | CAG-mRuby2-bGH | ComMAND base gene regulating mRuby2, CAG-driven |
| 235298 | CAG-mRuby2(miRE.FF4)-TS.FF4x1-bGH | ComMAND closed-loop circuit regulating mRuby2, CAG-driven |
| 235299 | EF1a-mGL-bGH | mGreenLantern expression to serve as a transfection marker, EF1a-driven |
| 235300 | EF1a-FXN-P2A-mRuby2-bGH | ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene) |
| 235302 | EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH | ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene) |
| 235303 | pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE | Lentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene) |
| 235304 | pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE | Lentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene) |
| 235305 | pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE | Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene) |
| 235306 | pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE | Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene) |
| 235307 | pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE | Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene) |
| 235308 | pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE | Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene) |
| 235309 | EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH | ComMAND open-loop circuit regulating mRuby2, EFS-driven |
| 235310 | EFS-mRuby2(miRE.FF4)-TS.FF4x1-bGH | ComMAND closed-loop circuit regulating mRuby2, EFS-driven |
| 235311 | hPGK-mRuby2(miRE.FF4)-TS.FF6x1-bGH | ComMAND open-loop circuit regulating mRuby2, hPGK-driven |
| 235312 | hPGK-mRuby2(miRE.FF4)-TS.FF4x1-bGH | ComMAND closed-loop circuit regulating mRuby2, hPGK-driven |
| 235313 | pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop) |
| 235314 | pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop) |
| 235315 | pLentiX1-[TRE3G-TS.FF6x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of mRuby2 with 5' microRNA target site only (open-loop) |
| 235316 | pLentiX1-[TRE3G-TS.FF4x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of mRuby2 with 5' microRNA target site only (closed-loop) |
| 235317 | pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of mRuby2 with intronic microRNA only |
| 235318 | pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of mRuby2 with 3' microRNA only |
| 235319 | pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of ComMAND open-loop circuit regulating mRuby2 |
| 235320 | pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2 |
| 235321 | pLentiX1-[TRE3G-TS.FF6x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 2 |
| 235322 | pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2 |
| 235323 | pLentiX1-[TRE3G-TS.FF6x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 3 |
| 235324 | pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE | Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3 |
| 235325 | pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA | PiggyBac integration vector for ComMAND base gene regulating mRuby2 |
| 235326 | pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA | PiggyBac integration vector for ComMAND open-loop circuit regulating mRuby2 |
| 235327 | pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA | PiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2 |