EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency.
Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, Iwasaki S, Takahashi K
Sci Adv. 2025 Apr 11;11(15):eadq5484. doi: 10.1126/sciadv.adq5484. Epub 2025 Apr 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236763 | PB-U6-CNCB | A piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-P2A-BSR. |
| 236764 | PB-2G-CNCB | A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-P2A-BSR. |
| 236768 | SB-CAG-mKate2-P2A-NANOG-IP | A sleeping beauty-based vector containing CAG promoter-driven mKate2-P2A-NANOG-IRES-Pac. |
| 236769 | SB-CAG-mCherry-P2A-EIF3D-IP | A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D-IRES-Pac. |
| 236770 | SB-CAG-mCherry-P2A-EIF3D-DD-IP | A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528D/S529D-IRES-Pac. |
| 236771 | SB-CAG-mCherry-P2A-EIF3D-NN-IP | A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528N/S529N-IRES-Pac. |
| 236772 | PB-TRE3G-CCRB | A piggybac-based cloning vector containing TRE3G promoter followed by cloning site and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD. |
| 237555 | PB-2G-CNCB_sgRBBP6-68 | A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-P2A-BSR. |