Protocol for CRISPR-mediated deletion of cis-regulatory element in murine Th17 cells for in vivo assessment of effector function.
Zhong X, Wu H, Wang G, Sun Z
STAR Protoc. 2025 Jun 20;6(2):103831. doi: 10.1016/j.xpro.2025.103831. Epub 2025 May 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237399 | MIGR1-U6gRNA1-Filler-v1 | For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v2. |
| 237400 | MIGR1-U6gRNA1-Filler-v2 | For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v1. |
| 237401 | MIGR1-U6gRNA2-Filler | For the sequential insertion of two guide RNAs into two U6gRNA cassettes. |