Optical profiling of autonomous Ca(2+) nanodomains generated by lysosomal TPC2 and TRPML1.
Davis LC, Morgan AJ, Galione A
Cell Calcium. 2023 Dec;116:102801. doi: 10.1016/j.ceca.2023.102801. Epub 2023 Sep 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240465 | TPC2-GCaMP6s | Lysosomal channel with a high-affinity GECI fused to the C-terminus |
| 240466 | TPC2-RCaMP181 | Lysosomal channel with a red, mid-affinity GECI fused to the C-terminus |
| 240467 | LAMP1-G-GECO1.2 | Mid-affinity GECI targeted to cytosolic face of the lysosome |
| 240468 | TPC2-mCherry | Red-tagged lysosomal channel |
| 240471 | G-GECO1.2 (F90L) | Low-affinity green GECI (cytosolic) |