Zaid Abassi Lab: Zaid Abassi Lab Plasmids 2025

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
240602	ACE2-HA-Linker-FLAG	Encodes doubly tagged ACE2 protein. HA on the N-terminal added after the signal peptide (a.a 1-17) and FLAG tag at the end of the insert. A linker sequence was added after the HA tag.
240603	ACE2-Mutant-C0	To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 located within the collectrin-like site on ACE2
240604	ACE2-Mutant-C1	To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 1 located within the collectrin-like site on ACE2
240606	ACE2-Mutant-R671A	To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2; R was substituted by A at Residue 671 of ACE2 in the collectrin-like site
240607	ACE2-Mutant-C3	To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 located within the collectrin-like site on ACE2
240609	ACE2-Mutant-DeltaC4	To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2 likely at Cluster 4 of ACE2 in the collectrin-like site. The whole C4 sequence was deleted in this mutant
240610	ACE2-Mutant-C(0+4)	To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 and Cluster 4 of ACE2
240611	ACE2-Mutant-C(3+4)	To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 and Cluster 4 of ACE2
240613	MasR-HA	Encodes MasR, a receptor on RAAS that antagonizes the AT1R physiological effects.
240614	AT1R-HA	Encodes AT1R, a key receptor on RAAS that regulated blood pressure