Zaid Abassi Lab: Zaid Abassi Lab Plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240602 | ACE2-HA-Linker-FLAG | Encodes doubly tagged ACE2 protein. HA on the N-terminal added after the signal peptide (a.a 1-17) and FLAG tag at the end of the insert. A linker sequence was added after the HA tag. |
| 240603 | ACE2-Mutant-C0 | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 located within the collectrin-like site on ACE2 |
| 240604 | ACE2-Mutant-C1 | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 1 located within the collectrin-like site on ACE2 |
| 240605 | ACE2-Mutant-R652A | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2; R was substituted by A at Residue 652 of ACE2 in the collectrin-like site |
| 240606 | ACE2-Mutant-R671A | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2; R was substituted by A at Residue 671 of ACE2 in the collectrin-like site |
| 240607 | ACE2-Mutant-C3 | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 located within the collectrin-like site on ACE2 |
| 240608 | ACE2-Mutant-C4 | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 4 located within the collectrin-like site on ACE2 |
| 240609 | ACE2-Mutant-DeltaC4 | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2 likely at Cluster 4 of ACE2 in the collectrin-like site. The whole C4 sequence was deleted in this mutant |
| 240610 | ACE2-Mutant-C(0+4) | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 and Cluster 4 of ACE2 |
| 240611 | ACE2-Mutant-C(3+4) | To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 and Cluster 4 of ACE2 |
| 240612 | TMPRSS2-Mutant-R255A | To test the cleavage of ACE2 by a non-autocleavable form of TMPRSS2 (Less active form) |
| 240613 | MasR-HA | Encodes MasR, a receptor on RAAS that antagonizes the AT1R physiological effects. |
| 240614 | AT1R-HA | Encodes AT1R, a key receptor on RAAS that regulated blood pressure |