Advancing knock-in approaches for robust genome editing in zebrafish
Rodriguez-Parks A, Beezley EG, Manna S, Silaban I, Almutawa SI, Cao S, Ahmed H, Guyer M, Baker S, Richards MP, Kang J
Biol Open 2026; bio.062472. doi: 10.1242/bio.062472
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200513 | pMC-ME_EGFP-pA | Mini circle middle entry plasmid for GFP-pA with flanking BsaI cut sites |
| 200514 | pMC-ME_2a-EGFP-pA ORF-1 with ATG | mini circle middle entry plasmid for 2a-eGFP-pA ORF1 (with ATG) with flanking BsaI cut sites |
| 200515 | pMC-ME_2a-EGFP-pA ORF-2 with ATG | mini circle middle entry plasmid for 2a-GFP-pA ORF 2 (with ATG) with flanking BsaI cut sites |
| 200516 | pMC-ME_2a-EGFP-pA ORF-3 with ATG | mini circle middle entry plasmid for 2a-GFP-pA-ORF 3 (with ATG) with flanking BsaI cut sites |
| 200517 | pMC-ME_2a-EGFP-pA ORF-1 without ATG | mini circle middle entry plasmid for 2a-eGFP-pA ORF1 (without ATG) with flanking BsaI cut sites |
| 200518 | pMC-ME_2a-EGFP-pA ORF-2 without ATG | mini circle middle entry plasmid for 2a-eGFP-pA ORF2 (without ATG) with flanking BsaI cut sites |
| 200519 | pMC-ME_2a-EGFP-pA ORF-3 without ATG | mini circle middle entry plasmid for 2a-eGFP-pA ORF3 (without ATG) with flanking BsaI cut sites |
| 200520 | pMC-ME_memb-EGFP-pA | Mini circle middle entry plasmid for membrane GFP- pA with flanking BsaI cut sites |
| 200521 | pMC-ME_mCherry-pA | Mini circle middle entry plasmid for mCherry- pA with flanking BsaI cut sites |
| 200522 | pMC-ME_2a-mCherry-pA ORF-1 with ATG | mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (with ATG) with flanking BsaI cut sites |
| 200523 | pMC-ME_2a-mCherry-pA ORF-2 with ATG | mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (with ATG) with flanking BsaI cut sites |
| 200524 | pMC-ME_2a-mCherry-pA ORF-3 with ATG | mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (with ATG) with flanking BsaI cut sites |
| 200525 | pMC-ME_2a-mCherry-pA ORF-1 without ATG | mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (without ATG) with flanking BsaI cut sites |
| 200526 | pMC-ME_2a-mCherry-pA ORF-2 without ATG | mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (without ATG) with flanking BsaI cut sites |
| 200527 | pMC-ME_2a-mCherry-pA ORF-3 without ATG | mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (without ATG) with flanking BsaI cut sites |
| 200528 | pMC-ME_memb-mCherry-pA | mini circle middle entry plasmid for membrane mCherry-pA with flanking BsaI cut sites |
| 200529 | pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1 | mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites |
| 200530 | pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2 | mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites |
| 200531 | pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3 | mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites |
| 200532 | pMC-ME mCherry-P2A-NTRv2-pA | mini circle middle entry plasmid for mCherry- P2A- ntr version 2- pA with flanking BsaI cut sites |
| 200533 | pMC-ME_BFP-pA | mini circle middle entry plasmid for BFP-pA with flanking BsaI cut sites |
| 200534 | pMC-ME_2a-BFP-pA ORF-1 without ATG | mini circle middle entry plasmid for 2a-BFP-pA ORF 1 (without ATG) with flanking BsaI cut sites |
| 200535 | pMC-ME_2a-BFP-pA ORF-2 without ATG | mini circle middle entry plasmid for 2a-BFP-pA ORF 2 (without ATG) with flanking BsaI cut sites |
| 200536 | pMC-ME_2a-BFP-pA ORF-3 without ATG | mini circle middle entry plasmid for 2a-BFP-pA ORF 3 (without ATG) with flanking BsaI cut sites |
| 200537 | pMC-ME_memb-BFP-pA | mini circle middle entry plasmid for membrane BFP-pA with flanking BsaI cut sites |
| 200538 | pMC-ME_BFP-P2A-NTRv2-pA | mini circle middle entry plasmid for BFP-P2A-NTR version 2-pA with flanking BsaI cut sites |
| 200539 | pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1 | mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites |
| 200540 | pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2 | mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites |
| 200541 | pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3 | mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites |
| 200542 | pMC-ME_CreER-pA | mini circle middle entry plasmid for CreER-pA with flanking BsaI cut sites |
| 200543 | pMC-ME_CreER-pA-FRT-acry:venus-pA-FRT | mini circle middle entry plasmid for CreER-pA-FRT-acry:venus-pA-FRT with flanking BsaI cut sites |
| 200544 | pMC-ME_CreER-pA-FRT-acry:mCherry-pA-FRT | mini circle middle entry plasmid for CreER-pA-FRT-acry: mCherry -pA-FRT with flanking BsaI cut sites |
| 200545 | pMC-ME_P2A-CreER-pA ORF1 FRT-acry:mCherry-pA-FRT | mini circle middle entry plasmid for P2A-CreER-pA (ORF 1) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites |
| 200546 | pMC-ME_P2A-CreER-pA ORF2 FRT-acry:mCherry-pA-FRT | mini circle middle entry plasmid for P2A-CreER-pA (ORF 2) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites |
| 200547 | pMC-ME_P2A-CreER-pA ORF3 FRT-acry:mCherry-pA-FRT | mini circle middle entry plasmid for P2A-CreER-pA (ORF 3) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites |
| 200550 | pMC-Dest-BsaI | Mini circle destination vector with BsaI sites for Golden Gate Assembly |
| 241150 | pMC-Dest-uni-gRNA5-BsaI | Destination vector for mini-Golden subcloning platform; contains universal sgRNA at 5' end |
| 241151 | EGFP-pA | Middle entry vector for mini-Golden subcloning platform; eGFP |
| 241152 | memb-EGFP-pA | Middle entry vector for mini-Golden subcloning platform; eGFP |
| 241153 | P2A-EGFP-pA-ORF-1 without ATG | Middle entry vector for mini-Golden subcloning platform; eGFP |
| 241154 | P2A-EGFP-pA-ORF-2 without ATG | Middle entry vector for mini-Golden subcloning platform; eGFP |
| 241155 | P2A-EGFP-pA-ORF-3 without ATG | Middle entry vector for mini-Golden subcloning platform; eGFP |
| 241156 | mCherry-pA | Middle entry vector for mini-Golden subcloning platform; mCherry |
| 241157 | memb-mCherry-pA | Middle entry vector for mini-Golden subcloning platform; mCherry |
| 241158 | P2A-mCherry-pA-ORF-1 without ATG | Middle entry vector for mini-Golden subcloning platform; mCherry |
| 241159 | P2A-mCherry-pA-ORF-2 without ATG | Middle entry vector for mini-Golden subcloning platform; mCherry |
| 241160 | P2A-mCherry-pA-ORF-3 without ATG | Middle entry vector for mini-Golden subcloning platform; mCherry |
| 241161 | memb-BFP-pA | Middle entry vector for mini-Golden subcloning platform; BFP |
| 241162 | pMC-ME_2a-BFP-ORF-1 without ATG | Middle entry vector for mini-Golden subcloning platform; BFP |
| 241163 | P2A-BFP-pA-ORF-2 without ATG | Middle entry vector for mini-Golden subcloning platform; BFP |
| 241164 | P2A-BFP-pA-ORF-3 without ATG | Middle entry vector for mini-Golden subcloning platform; BFP |
| 241165 | CreER-pA_FRT-acrymCherry | Middle entry vector for mini-Golden subcloning platform; creER; acry-mCherry |
| 241166 | CreER-pA_FRT-acry-venus | Middle entry vector for mini-Golden subcloning platform; creER; acry-venus |
| 241167 | P2A-CreER-pA-ORF1-FRT-acry mCherry | Middle entry vector for mini-Golden subcloning platform; creER |
| 241168 | P2A-CreER-pA-ORF2-FRT-acry mCherry | Middle entry vector for mini-Golden subcloning platform; creER |
| 241169 | P2A-CreER-pA-ORF3-FRT-acry mCherry | Middle entry vector for mini-Golden subcloning platform; creER |
| 241170 | MCS_BsmBI | Middle entry vector for mini-Golden subcloning platform; MCS; BsmBI |
| 241171 | pMid-TCGC-mNeongreen | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241172 | linker-P2A-lifeact-mNeongreen-ORF-1 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241173 | linker-P2A-lifeact-mNeongreen-ORF-2 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241174 | linker-P2A-lifeact-mNeongreen-ORF-3 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241175 | memb-mNeongreen | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241176 | linker-P2A-mNeongreen-ORF1 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241177 | linker-P2A-mNeongreen-ORF2 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241178 | linker-P2A-mNeongreen-ORF3 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241179 | FRT-acrymCherry-FRT | Middle entry vector for mini-Golden subcloning platform; acry-mCherry |
| 241180 | linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF1 | Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen |
| 241181 | linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF2 | Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen |
| 241182 | linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF3 | Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen |
| 241183 | linker-P2A-mem-mScarlet-ORF-1 | Middle entry vector for mini-Golden subcloning platform; mScarlet |
| 241184 | linker-P2A-mem-mScarlet-ORF-2 | Middle entry vector for mini-Golden subcloning platform; mScarlet |
| 241185 | linker-P2A-mem-mScarlet-ORF-3 | Middle entry vector for mini-Golden subcloning platform; mScarlet |
| 241186 | SA-linker-P2A-mem-mScarlet-ORF-1 | Middle entry vector for mini-Golden subcloning platform; mScarlet |
| 241187 | SA-linker-P2A-mem-mScarlet-ORF-2 | Middle entry vector for mini-Golden subcloning platform; mScarlet |
| 241188 | SA-linker-P2A-mem-mScarlet-ORF-3 | Middle entry vector for mini-Golden subcloning platform; mScarlet |
| 241189 | nzCas9n-GSG-linker-P2A-mNeongreen | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241190 | 3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen | Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen |
| 241191 | nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen | Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen |
| 241192 | linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF1 | Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen |
| 241193 | linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF2 | Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen |
| 241194 | linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF3 | Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen |
| 241195 | mCherry-P2A-NTRv2-pA | Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2 |
| 241196 | T2A-mCherry-P2A-NTRv2-pA-ORF-1 | Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2 |
| 241197 | T2A-mCherry-P2A-NTRv2-pA-ORF-2 | Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2 |
| 241198 | T2A-mCherry-P2A-NTRv2-pA-ORF-3 | Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2 |
| 241199 | T2A-BFP-P2A-NTRv2-pA-ORF-1 | Middle entry vector for mini-Golden subcloning platform; BFP; NTR-v2 |
| 241200 | T2A-BFP-P2A-NTRv2-pA-ORF-2 | Middle entry vector for mini-Golden subcloning platform; BFP; NTR-v2 |
| 241201 | T2A-BFP-P2A-NTRv2-pA-ORF-3 | Middle entry vector for mini-Golden subcloning platform; BFP; NTR-v2 |
| 241202 | lifeact-mNeongreen-pA | Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen |
| 241203 | SA-TCGC-mNeongreen | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241204 | SA-linker-P2A-lifeact-mNeongreen-ORF-1 | Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen |
| 241205 | SA-linker-P2A-lifeact-mNeongreen-ORF-2 | Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen |
| 241206 | SA-linker-P2A-lifeact-mNeongreen-ORF-3 | Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen |
| 241207 | SA-memb-mNeongreen | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241208 | SA-linker-P2A-mNeongreen-ORF1 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241209 | SA-linker-P2A-mNeongreen-ORF2 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241210 | SA-linker-P2A-mNeongreen-ORF3 | Middle entry vector for mini-Golden subcloning platform; mNeongreen |
| 241211 | SA-EGFP-pA | Middle entry vector for mini-Golden subcloning platform; eGFP |
| 241212 | SA-mCherry-pA | Middle entry vector for mini-Golden subcloning platform; mCherry |
| 241213 | pMC-dest-BsaI-cmlc2:mCherry | |
| 241214 | BsaI-MCS-cloning vector for golden gate |