Dissection of Galphas and Hedgehog Signaling Crosstalk Reveals Therapeutic Opportunities to Target Hedgehog-Dependent Tumors.
Krantz S, Bell BA, Lund K, Salinas Parra N, Ng Y, De Oliveira Rosa N, Mukhopadhyay S, St Croix B, Sarin KY, Weigert R, Raimondi F, Iglesias-Bartolome R
Cancer Res. 2025 Dec 29. doi: 10.1158/0008-5472.CAN-25-1109.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249268 | pCEFL_GPR161-HA | Expression of GPR161 wild type |
| 249269 | pCEFL_GPR161-HA_V129E | Expression of GPR161 V129E mutant |
| 249270 | pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1 | Expression of GLI1 |
| 249271 | pCEFL PRKACA HA WT | Expression of PKA (PRKACA) wild type |
| 249272 | pCEFL PRKACA HA T49I | Expression of PKA T49I mutant (PRKACAT49I) |
| 249273 | pCEFL PRKACA HA E128K | Expression of PKA T128K mutant (PRKACAE128K) |
| 249274 | pCEFL PRKACA HA R135W | Expression of PKA R135W mutant (PRKACAR135W) |
| 249275 | pCEFL PRKACA HA E171K | Expression of PKA E171K mutant (PRKACAE171K) |
| 249276 | pCEFL GNAS EE WT | Expression of Galphas (GNAS) wild type EE-tagged (internal) |
| 249277 | pCEFL GNAS EE K32R | Expression of Galphas K32R mutant (GNASK32R) EE-tagged (internal) |
| 249278 | pCEFL GNAS EE P116L | Expression of Galphas P116L mutant (GNASP116L) EE-tagged (internal) |
| 249279 | pCEFL GNAS EE N218I | Expression of Galphas N218I mutant (GNASN218I) EE-tagged (internal) |
| 249280 | pCEFL GNAS EE T350I | Expression of Galphas T350I mutant (GNAST350I) EE-tagged (internal) |
| 249281 | pGL4.R_8xGLIBS_minPro_Luc2p_Ren | Dual-luciferase GLI reporter plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression. |
| 249282 | pLenti X1 Puro 8xGLI_minPro_Luc2p_Ren | Dual-luciferase GLI reporter viral plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression. |