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Ramiro Iglesias-Bartolome Lab: Dissection of Galphas and Hedgehog signaling crosstalk reveals therapeutic opportunities to target adenosine receptor 2b in Hedgehog-dependent tumors.

Krantz S, Bell B, Lund K, Parra NS, Ng Y, De Oliveira Rosa N, Mukhopadhyay S, Croix BS, Sarin KY, Weigert R, Raimondi F, Iglesias-Bartolome R
bioRxiv [Preprint]. 2025 Feb 27:2025.02.21.639530. doi: 10.1101/2025.02.21.639530. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
249268pCEFL_GPR161-HAExpression of GPR161 wild type
249269pCEFL_GPR161-HA_V129EExpression of GPR161 V129E mutant
249281pGL4.R_8xGLIBS_minPro_Luc2p_RenDual-luciferase GLI reporter plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression.
249282pLenti X1 Puro 8xGLI_minPro_Luc2p_RenDual-luciferase GLI reporter viral plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression.

Antibodies from Article