Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster.
Vigano MA, Ell CM, Kustermann MMM, Aguilar G, Matsuda S, Zhao N, Stasevich TJ, Affolter M, Pyrowolakis G
Development. 2021 Mar 16;148(6):dev191700. doi: 10.1242/dev.191700.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244040 | mito_mCherry_SunTag | anchoring mCherry-1x SunTag to the mitochondrial membrane |
| 244041 | mito_mCherry_MoonTag | anchoring mCherry-1x gp41MoonTag to the mitochondrial membrane |
| 244043 | mito_mCherry_ALFA | anchoring mCherry-1x ALFA to the mitochondrial membrane |
| 244045 | H2B_mCherry_MoonTag | anchoring mCherry-1x MoonTag to the chromatin/nucleus |
| 244047 | H2B_mCherry_ALFA | anchoring mCherry-1x ALFAto the chromatin/nucleus |
| 244057 | Vimentin_mCherry_MoonTag | anchoring mCherry-1x MoonTag to the intermediate filaments |
| 244059 | Vimentin_mCherry_ALFA | anchoring mCherry-1x ALFA to the intermediate filaments |
| 244060 | mCherry_CD8_OLLAS_SunTag | anchoring 1xOLLAS-SunTag to membranes |
| 244061 | anti-GCN4_scFv_GFP | fusion of the SunTag binder (anti GCN4 scFv) to sfGFP |
| 244062 | anti-gp41_Nb_GFP | fusion of the MoonTag binder (nanobody2H10) to sfGFP |
| 244063 | anti-ALFA_Nb_GFP | fusion of the ALFA binder (nanobody) to sfGFP |
| 244064 | anti-ALFA_Nb_mEGFP | fusion of the ALFA binder (nanobody) to mEGFP |