Decoding post-transcriptional regulatory networks by RNA-linked CRISPR screening in human cells.
Nugent PJ, Park H, Wladyka CL, Yelland JN, Sinha S, Chen KY, Bynum C, Quarterman G, Lee SC, Hsieh AC, Subramaniam AR
Nat Methods. 2025 Jun;22(6):1237-1246. doi: 10.1038/s41592-025-02702-6. Epub 2025 May 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228230 | pHPHS309 | GFP dropout backbone for sgRNA library cloning |
| 228236 | pHPHS853 | Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad |
| 228237 | pHPHS926 | Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad |
| 228238 | pHPHS927 | Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad |
| 228240 | pHPHS800 | Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination |
| 228356 | pHPHS232 | Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus |