WNT7A/B assemble a GPR124-RECK-LRP5/6 co-receptor complex to activate beta-catenin signaling in brain endothelial cells.

Heiden R, Hannig L, Kuo CJ, Ergun S, Braunger BM, Vallon M
J Biol Chem. 2025 Sep 4:110682. doi: 10.1016/j.jbc.2025.110682. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
246547	pX459-Gpr124 gRNA	CRISPR vector co-expressing Cas9 and a mouse Gpr124 gRNA
246548	pX459-Lrp5 gRNA	CRISPR vector co-expressing Cas9 and a mouse Lrp5 gRNA
246549	pX459-Lrp6 gRNA	CRISPR vector co-expressing Cas9 and a mouse Lrp6 gRNA
246550	pX459-Fzd1/2/4/5/7/8 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd1/2/4/5/7/8 gRNA (conserved region)
246551	pX459-Fzd9 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd9 gRNA
246552	pX459-Fzd3 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd3 gRNA
246553	pX459-Fzd6 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd6 gRNA
246554	pX459-Fzd10 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd10 gRNA
246555	pX459-Dvl1/3 gRNA	CRISPR vector co-expressing Cas9 and a mouse Dvl1/3 gRNA (conserved region)
246556	pX459-Dvl2 gRNA	CRISPR vector co-expressing Cas9 and a mouse Dvl2 gRNA
246557	pBABE-Puro-BirA	Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag
246558	pCW-BAP-RECK-Blast	Lentiviral vector for inducible expression of human RECK (all-in-one Tet-On system), N-terminal BAP tag
246560	pBABE-Puro-EGFP	Retroviral expression vector for EGFP
246561	pCW-EGFP-Blast	Lentiviral vector for inducible expression of EGFP (all-in-one Tet-On system)
246562	pX459-Fzd1/2 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd1/2 gRNA (conserved region)
246563	pX459-Fzd4 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd4 gRNA
246564	pX459-Fzd5 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd5 gRNA
246565	pX459-Fzd8 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd8 gRNA
246566	pX459-Fzd7 gRNA	CRISPR vector co-expressing Cas9 and a mouse Fzd7 gRNA
246567	pX459-Flrt2 gRNA	CRISPR vector co-expressing Cas9 and a mouse Flrt2 gRNA
246568	pX459-Adam10 gRNA	CRISPR vector co-expressing Cas9 and a mouse Adam10 gRNA
246569	pX459-Tmem2 gRNA	CRISPR vector co-expressing Cas9 and a mouse Tmem2 gRNA
246570	pX459-Insr gRNA	CRISPR vector co-expressing Cas9 and a mouse Insr gRNA
246571	pX459-Cd109 gRNA	CRISPR vector co-expressing Cas9 and a mouse Cd109 gRNA
246572	pX459-Arf1 gRNA	CRISPR vector co-expressing Cas9 and a mouse Arf1 gRNA
246573	pX459-Itga5 gRNA	CRISPR vector co-expressing Cas9 and a mouse Itga5 gRNA
246574	pX459-Plxna1 gRNA	CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA
246575	pX459-Neo1 gRNA	CRISPR vector co-expressing Cas9 and a mouse Neo1 gRNA
246576	pX459-Itgav gRNA	CRISPR vector co-expressing Cas9 and a mouse Itgav gRNA
246577	pX459-Itgb1 gRNA	CRISPR vector co-expressing Cas9 and a mouse Itgb1 gRNA
246578	pX459-Nrp1 gRNA	CRISPR vector co-expressing Cas9 and a mouse Nrp1 gRNA