Site-specific DNA insertion into the human genome with engineered recombinases.
Fanton A, Bartie LJ, Martins JQ, Tran VQ, Goudy L, Kernick C, Durrant MG, Wei J, Armour-Garb Z, Pawluk A, Konermann S, Marson A, Gilbert LA, Roth TL, Hsu PD
Nat Biotechnol. 2025 Nov 6. doi: 10.1038/s41587-025-02895-3.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247151 | pEf1a-Dn29-P2A-GFP | Mammalian expression of a human codon optimized wildtype Dn29 recombinase |
| 247152 | pEf1a-superDn29-P2A-GFP | Mammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant) |
| 247153 | pEf1a-goldDn29-P2A-GFP | Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant) |
| 247154 | pEf1a-hifiDn29-P2A-GFP | Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant) |
| 247155 | pEf1a-Dn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3 |
| 247156 | pEf1a-superDn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3 |
| 247157 | pEf1a-goldDn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3 |
| 247158 | pEf1a-hifiDn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3 |
| 247159 | pDonor_attP-Ef1a-Puro-P2A-mCherry | attP donor plasmid expressing puromycin resistance and mCherry |
| 247160 | pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry | Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3 |
| 247161 | pIVT_Dn29-P2A-GFP | In vitro transcription of human codon optimized wildtype Dn29-P2A-GFP |
| 247162 | pIVT_superDn29-P2A-GFP | In vitro transcription of human codon optimized engineered superDn29-P2A-GFP |
| 247163 | pIVT_goldDn29-P2A-GFP | In vitro transcription of human codon optimized engineered goldDn29-P2A-GFP |
| 247164 | pIVT_hifiDn29-P2A-GFP | In vitro transcription of human codon optimized engineered hifiDn29-P2A-GFP |
| 247165 | pIVT_Dn29-dCas9-P2A-GFP | In vitro transcription of human codon optimized fusion of Dn29-dCas9-P2A-GFP |
| 247166 | pIVT_superDn29-dCas9-P2A-GFP | In vitro transcription of human codon optimized fusion of superDn29-dCas9-P2A-GFP |
| 247167 | pIVT_goldDn29-dCas9-P2A-GFP | In vitro transcription of human codon optimized fusion of goldDn29-dCas9-P2A-GFP |
| 247168 | pIVT_hifiDn29-dCas9-P2A-GFP | In vitro transcription of human codon optimized fusion of hifiDn29-dCas9-P2A-GFP |
| 247169 | pU6_H1-g3 | U6 driven gRNA expression of H1-g3 for targeting Dn29 attH1 |