Site-specific DNA insertion into the human genome with engineered recombinases
Fanton A, Bartie LJ, Martins JQ, Tran VQ, Goudy L, Durrant MG, Wei J, Pawluk A, Konermann S, Marson A, Gilbert LA, Hsu PD
bioRxiv 2024.11.01.621560
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247151 | pEf1a-Dn29-P2A-GFP | Mammalian expression of a human codon optimized wildtype Dn29 recombinase |
| 247152 | pEf1a-superDn29-P2A-GFP | Mammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant) |
| 247153 | pEf1a-goldDn29-P2A-GFP | Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant) |
| 247154 | pEf1a-hifiDn29-P2A-GFP | Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant) |
| 247155 | pEf1a-Dn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3 |
| 247156 | pEf1a-superDn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3 |
| 247157 | pEf1a-goldDn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3 |
| 247158 | pEf1a-hifiDn29-dCas9-P2A-GFP | Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3 |
| 247169 | pU6_H1-g3 | U6 driven gRNA expression of H1-g3 for targeting Dn29 attH1 |