Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. PubMed Article
Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52940 | HL 932 | MG1655 + ΔlacI |
| 52941 | HL 1255 | MG1655 + fimS (off orientation)::gfp::Asp terminator::CamR at fimA |
| 52942 | HL 1285 | MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA |
| 52943 | HL 4195 | MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(partial) |
| 52944 | HL 4233 | ΔfimB + ΔfimE + ΔfimA |
| 52946 | HL 6139 | MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(stop codon) |
| 52947 | HL 6166 | MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(IRL) |
| 52949 | HL 6279 | MG1655 + fimS (off orientation)::gfpAAV tag::Asp terminator::CamR at fimA + ΔlacI + KanR replaces fimB and fimE(partial) |
| 53014 | pHL 246 | AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1 |
| 53016 | pHL 651 | LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997). |
| 53017 | pHL 1277 | AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1 |
| 53018 | pHL 1278 | AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1 |
| 53019 | pHL 1454 | AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1 |
| 53020 | pHL 1903 | AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1 |
| 53023 | pHL 1916 | AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1 |
| 53024 | pHL 1917 | AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1 |