Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells.

Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di Ventura B
Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
61342	pDB22	Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)
61343	pDN34	mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)
61344	pDN41	mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)
61345	pDN42	mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)
61346	pDN43	mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)
61347	pDN77	mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)
61349	pDN80	LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.