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Eric Schirmer Lab: Schirmer lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
61991NET43 pEGFP-N2 (609)mammalian expression of nuclear envelope transmembrane protein
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61995NET92 pmRFP-N2 (643)mammalian expression of nuclear envelope transmembrane protein
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62045NET29A.Mm pEGFP-N2 (1326)mammalian expression of nuclear envelope transmembrane protein
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62046NET29b.Mm pEGFP-N2 (1327)mammalian expression of nuclear envelope transmembrane protein
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62052NET29A.Hs pRRLSIN (1742)lentiviral expression of nuclear envelope transmembrane protein
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62053NET29B.mm pRRLSIN (1744)lentiviral expression of nuclear envelope transmembrane protein
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124262STING-V1C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
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124263V1-STINGN-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
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124264STING-V2C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
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124265V2-STINGN-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
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124266NLS-mRFP-V1Nuclear localised mRFP reporter construct with one half of split Venus
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124267NLS-mRFP-V2Nuclear localised mRFP reporter construct with one half of split Venus
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124268 Lamin A-mRFPWild-type Lamin A, C-terminally tagged with mRFP
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124269Lamin A (R298C)-mRFPLamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.
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124270Lamin A (R377H)-mRFPLamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
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124271Lamin A (G465D)-mRFPLamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
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124272Lamin A (R527P)-mRFPLamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
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124273Lamin A (R482Q)-mRFPLamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
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124274Lamin A (R435C)-mRFPLamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.
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