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"" CRISPR Fly Design


Background

CRISPR fly design is a genome engineering project in Drosophila, consisting of transgenic cas9 fly lines and gRNA plasmids. Project tools can be used for both generating knockouts and precise modifications using HDR.

For more information about this project, please refer to the CRISPR Fly Design homepage.

gRNA plasmids

ID Name Description PI
49408 pCFD1-dU6:1gRNA expression of gRNA under control of the Drosophila U6:1 promoter Bullock
49409 pCFD2-dU6:2gRNA expression of gRNA under control of the Drosophila U6:2 promoter Bullock
49410 pCFD3-dU6:3gRNA expression of gRNA under control of the Drosophila U6:3 promoter Bullock
49411 pCFD4-U6:1_U6:3tandemgRNAs expression of two gRNAs from Drosophila U6:1 and U6:3 Bullock
73914 pCFD5 multiplex gRNA plasmid Bullock
73915 pCFD6 tissue-specific multiplex gRNA plasmid Bullock

Cas9 plasmids

ID Name Description PI
62209 pAct-Cas9 Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. Bullock
62208 pnos-Cas9-nos Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster. Bullock
62211 pAct-Fok1:dCas9 Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster. Bullock
62210 pnos-Fok1:dCas9-nos Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster. Bullock