CRISPR Fly Design

Background

CRISPR fly design is a genome engineering project in Drosophila, consisting of transgenic cas9 fly lines and gRNA plasmids. Project tools can be used for both generating knockouts and precise modifications using HDR.

For more information about this project, please refer to the CRISPR Fly Design homepage.

gRNA plasmids

ID	Name	Description	PI
49408	pCFD1-dU6:1gRNA	expression of gRNA under control of the Drosophila U6:1 promoter	Bullock
49409	pCFD2-dU6:2gRNA	expression of gRNA under control of the Drosophila U6:2 promoter	Bullock
49410	pCFD3-dU6:3gRNA	expression of gRNA under control of the Drosophila U6:3 promoter	Bullock
49411	pCFD4-U6:1_U6:3tandemgRNAs	expression of two gRNAs from Drosophila U6:1 and U6:3	Bullock
73914	pCFD5	multiplex gRNA plasmid	Bullock
73915	pCFD6	tissue-specific multiplex gRNA plasmid	Bullock

Cas9 plasmids

ID	Name	Description	PI
62209	pAct-Cas9	Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.	Bullock
62208	pnos-Cas9-nos	Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.	Bullock
62211	pAct-Fok1:dCas9	Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.	Bullock
62210	pnos-Fok1:dCas9-nos	Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.	Bullock