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Huang Lab CRISPR Plasmids Available from Addgene

A human codon-optimized Cas9 nuclease expression plasmid. The arrangement of the Nuclear Localization Signal (NLS) relative to the hCas9 coding region was optimized for the most efficient localization of the nuclease. This optimized hCas9 expression has been shown to be functional in zebrafish embryos and mouse embryos.

These plasmids are described in:

Generation of gene-modified mice via Cas9/RNA-mediated gene targeting. Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X. Cell Res. 2013 Apr 2. PubMed PMID 23545779. PMC3641603.

Individual plasmids can be ordered via the links below:

ID Plasmid
44758 pST1374-NLS-flag-linker-Cas9: A human codon-optimized Cas9 expression plasmid, specifically designed for efficient nuclear localization in eukaryotes.