CRISPR Plasmids: Parasites
Eukaryotic parasites continue to cause substantial death and disease in the world. Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania (leishmaniasis), and others have been the focus of biomedical research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some of these parasites. Together, these tools have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission, and development. This collection brings together some of the plasmids that have been used in these efforts.
Addgene's parasite CRISPR plasmid collection also includes the Toxoplasma CRISPR Knockout Pooled Library, a Toxoplasma gondii genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Parasite".
The content for this page was generated with the help of Scott Lindner.
Cut
| ID | Plasmid | Gene/Insert | Selectable Marker | PI | Publication |
|---|
CRISPR Resources
Addgene has a large selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page. Find a comprehensive list of CRISPR resources by visiting our CRISPR reference page.
Content last reviewed: 9 December 2025
Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!