Chromoprotein kit 1.0
(Kit #
1000000174
)
Depositing Lab: Anthony Forster
A synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.
This kit will be sent as individual bacterial stabs at room temperature.
Original Publication
Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. PubMed PMID: 29760772
Description
This collection deposited by Anthony Forster's lab contains a set of 11 Biobrick™ genetic modules for standardized 3A assembly. It comprises plasmids for a published teaching course (see the Protocols & Resources tab) where successful coupling of two modules causes visible chromoprotein expression in E. coli. Also included are plasmids that constitutively express other chromoproteins.
Biobrick™ “3A” assembly standard. Image based on Fig. 25 of Liljeruhm J, Gullberg E & Forster AC (2014). Synthetic biology: a lab manual. Lab Manual
Additional Protocols
Synthetic biology: a lab manual. Liljeruhm J, Gullberg E, Forster AC. World Scientific Press (Singapore) 2014, 204 pp. doi: 10.1142/9061. Lab Manual
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“Chromoprotein kit 1.0 was made available via the Forster lab (Addgene kit #1000000174).”
For your Reference section:
Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. PubMed PMID: 29760772
The Chromoprotein kit 1.0 contains 11 plasmids, sent as bacterail stabs of the E. coli strain DH5alpha, found in the four categories below. Please refer to the individual plasmid pages below for more details on each plasmid in this kit:
Promoter (including RFP reporter)
Chromoprotein gene (including RBS, not promoter)
- asPink chromoprotein promoter-less
- amilCP chromoprotein promoter-less
- amilGFP chromoprotein promoter-less
Chromoprotein expression plasmids (including promoter and RBS in pSB1C3 vector)
- aeBlue chromoprotein
- amajLime chromoprotein
- tsPurple chromoprotein
- amilCP_Orange chromoprotein
- amilCP_Pink chromoprotein
Vector backbone (including RFP reporter)
