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Chromoprotein kit 1.0
(Kit # 1000000174 )

Depositing Lab:   Anthony Forster

A synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.

This kit will be sent as individual bacterial stabs at room temperature.

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$400 USD + shipping

Available to academics and nonprofits only.

Original Publication

Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. PubMed PMID: 29760772

Description

This collection deposited by Anthony Forster's lab contains a set of 11 Biobrick™ genetic modules for standardized 3A assembly. It comprises plasmids for a published teaching course (see the Protocols & Resources tab) where successful coupling of two modules causes visible chromoprotein expression in E. coli. Also included are plasmids that constitutively express other chromoproteins.

Chromoprotein kit 1.0.jpg

Biobrick™ “3A” assembly standard. Image based on Fig. 25 of Liljeruhm J, Gullberg E & Forster AC (2014). Synthetic biology: a lab manual. Lab Manual

Additional Protocols

Synthetic biology: a lab manual. Liljeruhm J, Gullberg E, Forster AC. World Scientific Press (Singapore) 2014, 204 pp. doi: 10.1142/9061. Lab Manual

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“Chromoprotein kit 1.0 was made available via the Forster lab (Addgene kit #1000000174).”

For your Reference section:

Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. PubMed PMID: 29760772

The Chromoprotein kit 1.0 contains 11 plasmids, sent as bacterail stabs of the E. coli strain DH5alpha, found in the four categories below. Please refer to the individual plasmid pages below for more details on each plasmid in this kit:

Promoter (including RFP reporter)

Chromoprotein gene (including RBS, not promoter)

Chromoprotein expression plasmids (including promoter and RBS in pSB1C3 vector)

Vector backbone (including RFP reporter)

chromoprotein stabs.jpg
Chromprotein expression in bacterial stabs (aeBlue, amajLime, tsPurple, amilCP_Orange, amilCP_Pink).
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