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C. elegans miniMos Transgenesis Kit
(Kit # 1000000031 )

Depositing Lab:   Erik Jorgensen

This 29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$500 USD + shipping

Available to academics and nonprofits only.

Original Publication

The technique has been validated extensively and the Jorgensen lab has therefore decided to release the reagents prior to publication. Please cite "C. Frøkjær-Jensen and E.M. Jorgensen, unpublished reagents" if you use the reagents in a publication before we publish the method.

Description

The miniMos transposon is a modified Mos1 transposon that can be used to generate transgenic nematodes, including C. elegans and C. briggsae . The Mos1 transposon has been modified to allow transposition with cargo at high frequency and at high fidelity. Transgenes inserted into the miniMos transposon can be integrated in the genome as a single copy by injection or by heat-shock. Insertions are selected for with genetic markers ( unc-119 ) or antibiotic markers (NeoR or PuroR) and the exact insertion site can be determined by inverse PCR.

miniMOS.png

This kit consists of cloning vectors that are compatible with three-fragment Gateway cloning and with standard restriction cloning into multiple-cloning sites. The kit includes co-injection markers (Mos1 transposase helper plasmid, negative selection marker peel-1 and mCherry fluorescent markers), cloning vectors, a recombineering cassette and a set of positive control vectors.

Kit Documentation

Icon Jorgensen miniMos Transgenesis kit (274.6 KB)

Resources

More information on these plasmids and kit, as well as protocols for insertion and mapping insertions, can be found at Wormbuilder.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit used for generation of transgenic nematodes was a gift from C. Frøkjær-Jensen and E.M. Jorgensen (Addgene kit # 1000000031)"

C. elegans miniMos transgenesis kit - #1000000031

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Chloramphenicol and Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pCFJ909
Ampicillin
A / 2 pCFJ910
Ampicillin
A / 3 pCFJ1201
Ampicillin
A / 4 pCFJ1202
Ampicillin
A / 5 pCFJ1200
Ampicillin
A / 6 pCFJ1272
Ampicillin
A / 7 pCFJ1273
Ampicillin
A / 8 pCFJ496
Ampicillin
A / 9 pCFJ914
Ampicillin
A / 10 pCFJ1208
Ampicillin
A / 11 pCFJ1209
Ampicillin
A / 12 pCFJ1324
Ampicillin
B / 2 pCFJ420 - Peft-3::GFP::H2B (ubiquitous)
Ampicillin
B / 3 pCFJ421 - Pmyo-2::GFP::H2B (pharynx)
Ampicillin
B / 4 pCFJ601 - Peft-3 Mos1 transposase
Ampicillin
B / 5 pMA122 - peel-1 negative selection
Ampicillin
B / 6 pGH8 - pRAB-3::mCherry::unc-54utr
Ampicillin
B / 7 pCFJ90 - Pmyo-2::mCherry::unc-54utr
Ampicillin
B / 8 pCFJ104 - Pmyo-3::mCherry::unc-54
Ampicillin
B / 9 pJL44 - Phsp16.48::MosTase::glh-2utr
Ampicillin
B / 10 pCFJ906
Chloramphenicol and Ampicillin
B / 11 pCFJ907
Chloramphenicol and Ampicillin
B / 12 pCFJ908
Chloramphenicol and Ampicillin
C / 1 pCFJ1000
Chloramphenicol and Ampicillin
C / 2 pCFJ1001
Chloramphenicol and Ampicillin
C / 3 pCFJ1002
Chloramphenicol and Ampicillin
C / 4 pCFJ1258
Chloramphenicol and Ampicillin
C / 5 pCFJ1259
Chloramphenicol and Ampicillin
Data calculated @ 2017-05-22

Kit Plate Map - #1000000031

Plate #1

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A
B
C
D
E
F
G
H
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