C. elegans miniMos Transgenesis Kit
Depositing Lab: Erik Jorgensen
This 28 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae.
This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
The technique has been validated extensively and the Jorgensen lab has therefore decided to release the reagents prior to publication. Please cite "C. Frøkjær-Jensen and E.M. Jorgensen, unpublished reagents" if you use the reagents in a publication before we publish the method.
The miniMos transposon is a modified Mos1 transposon that can be used to generate transgenic nematodes, including C. elegans and C. briggsae . The Mos1 transposon has been modified to allow transposition with cargo at high frequency and at high fidelity. Transgenes inserted into the miniMos transposon can be integrated in the genome as a single copy by injection or by heat-shock. Insertions are selected for with genetic markers ( unc-119 ) or antibiotic markers (NeoR or PuroR) and the exact insertion site can be determined by inverse PCR.
This kit consists of cloning vectors that are compatible with three-fragment Gateway cloning and with standard restriction cloning into multiple-cloning sites. The kit includes co-injection markers (Mos1 transposase helper plasmid, negative selection marker peel-1 and mCherry fluorescent markers), cloning vectors, a recombineering cassette and a set of positive control vectors.
More information on these plasmids and kit, as well as protocols for insertion and mapping insertions, can be found at Wormbuilder.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The plasmid kit used for generation of transgenic nematodes was a gift from C. Frøkjær-Jensen and E.M. Jorgensen (Addgene kit # 1000000031)"
C. elegans miniMos transgenesis kit - #1000000031
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
|Chloramphenicol and Ampicillin|
|A / 1||pCFJ909||Ampicillin|
|A / 2||pCFJ910||Ampicillin|
|A / 3||pCFJ1201||Ampicillin|
|A / 4||pCFJ1202||Ampicillin|
|A / 5||pCFJ1200||Ampicillin|
|A / 6||pCFJ1272||Ampicillin|
|A / 7||pCFJ1273||Ampicillin|
|A / 8||pCFJ496||Ampicillin|
|A / 9||pCFJ914||Ampicillin|
|A / 10||pCFJ1208||Ampicillin|
|A / 11||pCFJ1209||Ampicillin|
|A / 12||pCFJ1324||Ampicillin|
|B / 2||pCFJ420 - Peft-3::GFP::H2B (ubiquitous)||Ampicillin|
|B / 3||pCFJ421 - Pmyo-2::GFP::H2B (pharynx)||Ampicillin|
|B / 4||pCFJ601 - Peft-3 Mos1 transposase||Ampicillin|
|B / 5||pMA122 - peel-1 negative selection||Ampicillin|
|B / 6||pGH8 - pRAB-3::mCherry::unc-54utr||Ampicillin|
|B / 7||pCFJ90 - Pmyo-2::mCherry::unc-54utr||Ampicillin|
|B / 8||pCFJ104 - Pmyo-3::mCherry::unc-54||Ampicillin|
|B / 9||pJL44 - Phsp16.48::MosTase::glh-2utr||Ampicillin|
|B / 10||pCFJ906||Chloramphenicol and Ampicillin|
|B / 11||pCFJ907||Chloramphenicol and Ampicillin|
|B / 12||pCFJ908||Chloramphenicol and Ampicillin|
|C / 1||pCFJ1000||Chloramphenicol and Ampicillin|
|C / 2||pCFJ1001||Chloramphenicol and Ampicillin|
|C / 3||pCFJ1002||Chloramphenicol and Ampicillin|
|C / 4||pCFJ1258||Chloramphenicol and Ampicillin|
|C / 5||pCFJ1259||Chloramphenicol and Ampicillin|