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Open Source Wnt Project Wnt Logo

Open Source Wnt Project Plasmids
(Kit # 1000000022 )

Depositing Labs:   Marian Waterman, David Virshup

The Open Source Wnt Kit is a 95-plasmid library of all 19 Wnt clones. The collection contains full-length open reading frames (ORFs) for every human Wnt ligand.

This kit consists of 1 plate, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$375 USD + shipping

Available to academics and nonprofits only.

Original Publication

A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation . 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PubMed .

Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J. Biol. Chem. . 2014 May 19; doi:10.1074/jbc.M114.575027 PubMed .

Description

The Open Source Wnt Project was initiated to create a collection of standardized Wnt plasmids that are easily accessible to the research community. The project and plasmids were created by Dr. Marian Waterman's group at UC Irvine and Dr. David Virshup's group at Duke Singapore as a way to pool community resources and efforts to develop an accessible and affordable set of reagents for the Wnt community. The Open Source Wnt Kit is a 95-plasmid library of all 19 Wnt clones. The collection contains full-length open reading frames (ORFs) for every human Wnt ligand.

In addition to this original set of 76 clones, Dr. Bryan MacDonald and Dr. Xi He have contributed a modified version of each tagged Wnt that enables epitope tagging without loss of Wnt signaling activity.

Read Addgene's Septemeber 2013 interview with Marian Waterman, Crowd-Sourced Science: The Open Source Wnt Project .

Kit Documentation

Icon Information and Plate Map PDF (96.8 KB)

Resources

Icon Open Source Wnt Info Sheet (94.4 KB)

Icon Open source Wnt Nomenclature (42.5 KB)

Icon Wnts in pcDNA3.2 V5-DEST Map (4.5 MB)

Icon Wnts in pENTR D-TOPO Map (1.9 MB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

"The plasmid kit used in this publication was a gift from Marian Waterman, David Virshup and Xi He (Addgene kit # 1000000022)"

For your Reference section:

A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation . 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PubMed .

Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J. Biol. Chem. . 2014 May 19; doi:10.1074/jbc.M114.575027 PubMed .

Wnt Open Source - #1000000022

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Kanamycin
Ampicillin

Inventory

Well Plasmid Resistance
A / 1 Wnt1 STOP
Kanamycin
A / 2 Wnt2 STOP
Kanamycin
A / 3 Wnt2B2 STOP
Kanamycin
A / 4 Wnt3A STOP
Kanamycin
A / 5 Wnt3 STOP
Kanamycin
A / 6 Wnt4 STOP
Kanamycin
A / 7 Wnt5A STOP
Kanamycin
A / 8 Wnt5B STOP
Kanamycin
A / 9 Wnt6 STOP
Kanamycin
A / 10 Wnt7A STOP
Kanamycin
A / 11 Wnt7B STOP
Kanamycin
A / 12 Wnt8A STOP
Kanamycin
B / 1 Wnt8B STOP
Kanamycin
B / 2 Wnt9A STOP
Kanamycin
B / 3 Wnt9B STOP
Kanamycin
B / 4 Wnt10A STOP
Kanamycin
B / 5 Wnt10B STOP
Kanamycin
B / 6 Wnt11 STOP
Kanamycin
B / 7 Wnt16 STOP
Kanamycin
B / 8 Wnt1 Non-STOP
Kanamycin
B / 9 Wnt2 Non-STOP
Kanamycin
B / 10 Wnt2B2 Non-STOP
Kanamycin
B / 11 Wnt3A Non-STOP
Kanamycin
B / 12 Wnt3 Non-STOP
Kanamycin
C / 1 Wnt4 Non-STOP
Kanamycin
C / 2 Wnt5A Non-STOP
Kanamycin
C / 3 Wnt5B Non-STOP
Kanamycin
C / 4 Wnt6 Non-STOP
Kanamycin
C / 5 Wnt7A Non-STOP
Kanamycin
C / 6 Wnt7B Non-STOP
Kanamycin
C / 7 Wnt8A Non-STOP
Kanamycin
C / 8 Wnt8B Non-STOP
Kanamycin
C / 9 Wnt9A Non-STOP
Kanamycin
C / 10 Wnt9B Non-STOP
Kanamycin
C / 11 Wnt10A Non-STOP
Kanamycin
C / 12 Wnt11 Non-STOP
Kanamycin
D / 1 Wnt16 Non-STOP
Kanamycin
D / 2 Wnt10B Non-STOP
Kanamycin
D / 3 pcDNA-Wnt2
Ampicillin
D / 4 pcDNA-Wnt2B2
Ampicillin
D / 5 pcDNA-Wnt3A
Ampicillin
D / 6 pcDNA-Wnt3
Ampicillin
D / 7 pcDNA-Wnt4
Ampicillin
D / 8 pcDNA-Wnt5A
Ampicillin
D / 9 pcDNA-Wnt5B
Ampicillin
D / 10 pcDNA-Wnt6
Ampicillin
D / 11 pcDNA-Wnt7A
Ampicillin
D / 12 pcDNA-Wnt7B
Ampicillin
E / 1 pcDNA-Wnt8A
Ampicillin
E / 2 pcDNA-Wnt8B
Ampicillin
E / 3 pcDNA-Wnt9A
Ampicillin
E / 4 pcDNA-Wnt9B
Ampicillin
E / 5 pcDNA-Wnt10A
Ampicillin
E / 6 pcDNA-Wnt10B
Ampicillin
E / 7 pcDNA-Wnt11
Ampicillin
E / 8 pcDNA-Wnt16
Ampicillin
E / 9 pcDNA-Wnt1-V5
Ampicillin
E / 10 pcDNA-Wnt2-V5
Ampicillin
E / 11 pcDNA-Wnt2B2-V5
Ampicillin
E / 12 pcDNA-Wnt3A-V5
Ampicillin
F / 1 pcDNA-Wnt3-V5
Ampicillin
F / 2 pcDNA-Wnt4-V5
Ampicillin
F / 3 pcDNA-Wnt5A-V5
Ampicillin
F / 4 pcDNA-Wnt5B-V5
Ampicillin
F / 5 pcDNA-Wnt6-V5
Ampicillin
F / 6 pcDNA-Wnt7A-V5
Ampicillin
F / 7 pcDNA-Wnt7B-V5
Ampicillin
F / 8 pcDNA-Wnt8A-V5
Ampicillin
F / 9 pcDNA-Wnt8B-V5
Ampicillin
F / 10 pcDNA-Wnt9A-V5
Ampicillin
F / 11 pcDNA-Wnt9B-V5
Ampicillin
F / 12 pcDNA-Wnt10A-V5
Ampicillin
G / 1 pcDNA-Wnt10B-V5
Ampicillin
G / 2 pcDNA-Wnt11-V5
Ampicillin
G / 3 pcDNA-Wnt16-V5
Ampicillin
G / 4 pcDNA-Wnt1
Ampicillin
G / 5 Active Wnt1-V5
Ampicillin
G / 6 Active Wnt2B-V5
Ampicillin
G / 7 Active Wnt2-V5
Ampicillin
G / 8 Active Wnt3A-V5
Ampicillin
G / 9 Active Wnt3-V5
Ampicillin
G / 10 Active Wnt4-V5
Ampicillin
G / 11 Active Wnt5A-V5
Ampicillin
G / 12 Active Wnt5B-V5
Ampicillin
H / 1 Active Wnt6-V5
Ampicillin
H / 2 Active Wnt7A-V5
Ampicillin
H / 3 Active Wnt7B-V5
Ampicillin
H / 4 Active Wnt8A-V5
Ampicillin
H / 5 Active Wnt8B-V5
Ampicillin
H / 6 Active Wnt9A-V5
Ampicillin
H / 7 Active Wnt9B-V5
Ampicillin
H / 8 Active Wnt10A-V5
Ampicillin
H / 9 Active Wnt10B-V5
Ampicillin
H / 10 Active Wnt11-V5
Ampicillin
H / 11 Active Wnt16-V5
Ampicillin
Data calculated @ 2016-12-06

Kit Plate Map - #1000000022

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