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Sabatini & Wood Cancer Pathways ORFs Kit
(Kit # 1000000072 )

Depositing Labs:   David Sabatini and Kris Wood

The Cancer Pathways ORFs Kit is a set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN, Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, McDonnell DP, Sabatini DM, Wood KC. Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877. PubMed PMID: 25538079 .

Description

This collection contains cDNAs that were used in a cancer cell screen that identified mutants that conferred resistance to anticancer agents. 17 signaling pathways were selected based on their frequent involvement in cancer cell survival, growth, apoptosis, and differentiation. Core pathway nodes of each pathway were identified and cDNAs were selected that constitutively activated or inactivated the pathway, when overexpressed. These constructs were then screened using anticancer drugs, with the goal of identifying the constructs that conferred resistance.

Depositor-provided Files

Icon Sabatini Wood Cancer Pathways ORF Kit Protocol (469.8 KB)

Additional Resources

Podcast with Kris Wood discussing this technology

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Cancer Pathways ORF Kit was a gift from David Sabatini and Kris Wood (Addgene kit # 1000000072).”

For your Reference section:

Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN, Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, McDonnell DP, Sabatini DM, Wood KC. Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877. PubMed PMID: 25538079 .

Cancer Pathways ORFs Kit - #1000000072

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
A / 1 Kras (G12V)-pcw107
Ampicillin
A / 2 Hras (G12V)-pcw107
Ampicillin
A / 3 MEK1 (S218D,S222D)-pcw107
Ampicillin
A / 4 myr-FLAG-PIK3CA-pcw107
Ampicillin
A / 5 myr-FLAG-AKT1-pcw107
Ampicillin
A / 6 FLAG-Rheb (Q64L)-pcw107
Ampicillin
A / 7 IKKalpha (S176E,S180E)-pcw107-V5
Ampicillin
A / 8 FLAG-IKKbeta (S177E,S181E)-pcw107
Ampicillin
A / 9 JAK2 (V617F)-pcw107-V5
Ampicillin
A / 10 Stat3 (A662C,N664C,V667L)-pcw107-V5
Ampicillin
A / 11 beta-catenin (S33A, S37A, T41A, S45A)-pcw107
Ampicillin
A / 12 beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
Ampicillin
B / 1 GSK3beta (K85A)-pcw107-V5
Ampicillin
B / 2 beta-catenin (S33Y)-pcw107
Ampicillin
B / 3 beta-catenin (S33Y)-pcw107-V5
Ampicillin
B / 4 JNK2 WT O/E (MAPK9)-pcw107-V5
Ampicillin
B / 5 FLAG-Mkk7-JNK2 fusion-pcw107
Ampicillin
B / 6 MEK5 DD(S311D,T315D)-pcw107-V5
Ampicillin
B / 7 myr-FLAG-MEK5-pcw107
Ampicillin
B / 8 Notch1 intracellular domain-pcw107
Ampicillin
B / 9 Notch1 intracellular domain-pcw107-V5
Ampicillin
B / 10 Notch3 intracellular domain-pcw107-V5
Ampicillin
B / 11 p38 WT O/E (MAPK14)-pcw107-V5
Ampicillin
B / 12 FLAG-MKK6 (S207E,T211E)-pcw107
Ampicillin
C / 1 Gli2 truncation-pcw107-V5
Ampicillin
C / 2 SmoM2 (W535L)-pcw107
Ampicillin
C / 3 SmoM2 (W535L)-pcw107-V5
Ampicillin
C / 4 TGFbetaR1 (T204D)-pcw107-V5
Ampicillin
C / 5 BCL2-pcw107-V5
Ampicillin
C / 6 BCL-XL-pcw107-V5
Ampicillin
C / 7 Caspase-8 (C360A)-pcw107-V5
Ampicillin
C / 8 Caspase-3 (C163A)-pcw107-V5
Ampicillin
C / 9 ERalpha (Y537S mutant)-pcw107-V5
Ampicillin
C / 10 AR-V7-pcw107
Ampicillin
C / 11 AR-V7-pcw107-V5
Ampicillin
C / 12 FLAG-YAP2 (8SA)-pcw107
Ampicillin
D / 1 p53 (dominant negative R175H mutant)-pcw107-V5
Ampicillin
D / 2 HRas (G12V, E37G)-pcw107
Ampicillin
D / 3 HRas (G12V, E37G)-pcw107-V5
Ampicillin
D / 4 Rgl2-CAAX-pcw107-V5
Ampicillin
D / 5 RalA (G23V)(mature peptide)-pcw107
Ampicillin
D / 6 RalA (G23V)(mature peptide)-pcw107-V5
Ampicillin
D / 7 RalA (G23V)( full )-pcw107
Ampicillin
D / 8 RalA (G23V)( full )-pcw107-V5
Ampicillin
D / 9 HcRed-pcw107
Ampicillin
D / 10 HcRed-pcw107-V5
Ampicillin
D / 11 Luciferase-pcw107
Ampicillin
D / 12 Luciferase-pcw107-V5
Ampicillin
E / 1 MEK1-pcw107-V5
Ampicillin
Data cached @ 2017-11-24

Kit Plate Map - #1000000072

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