Retroviral Barcoding Library
(Kit #
1000000097
)
Depositing Lab: Monte Winslow
The Winslow lab barcoding library consists of 96 retroviral plasmids, each with a unique 6 nucleotide barcode.
This collection can be used for in vivo phenotypic screening of small-molecule libraries and can be combined with high-throughput sequencing to perform multiplexed analysis of compound pretreated cells.
Original Publication
An in vivo multiplexed small-molecule screening platform. Grüner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, Winslow MM. Nat Methods. 2016 Sep 12.; doi: 10.1038/nmeth.3992. PubMed PMID: 27617390.
Description
Example schematic of 6-nucleotide barcode system. Vector backbone is retroviral MSCV-BC-GFP/Puro.
Supplemental Figure 1G. Reprinted by permission from Macmillan Publishers Ltd: Nature Methods Grüner and Schulze et al., copyright 2016.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“Retroviral Barcoding Library was a gift from Monte Winslow (Addgene kit #1000000097).”
For your Reference section:
An in vivo multiplexed small-molecule screening platform. Grüner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, Winslow MM. Nat Methods. 2016 Sep 12.; doi: 10.1038/nmeth.3992. PubMed PMID: 27617390.
Winslow Lab Retroviral Barcoding Library - #1000000097
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin |