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Retroviral Barcoding Library
(Kit # 1000000097 )

Depositing Lab:  Monte Winslow

The Winslow lab barcoding library consists of 96 retroviral plasmids, each with a unique 6 nucleotide barcode.

This collection can be used for in vivo phenotypic screening of small-molecule libraries and can be combined with high-throughput sequencing to perform multiplexed analysis of compound pretreated cells.

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Original Publication

An in vivo multiplexed small-molecule screening platform. Grüner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, Winslow MM. Nat Methods. 2016 Sep 12.; doi: 10.1038/nmeth.3992. PubMed PMID: 27617390.

Description

Example schematic of 6-nucleotide barcode system. Vector backbone is retroviral MSCV-BC-GFP/Puro.

Supplemental Figure 1G. Reprinted by permission from Macmillan Publishers Ltd: Nature Methods Grüner and Schulze et al., copyright 2016.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“Retroviral Barcoding Library was a gift from Monte Winslow (Addgene kit #1000000097).”

For your Reference section:

An in vivo multiplexed small-molecule screening platform. Grüner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, Winslow MM. Nat Methods. 2016 Sep 12.; doi: 10.1038/nmeth.3992. PubMed PMID: 27617390.

Winslow Lab Retroviral Barcoding Library - #1000000097

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

 Ampicillin

Inventory

Well Plasmid Resistance
A / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode1
Ampicillin
A / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode9
Ampicillin
A / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode17
Ampicillin
A / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode25
Ampicillin
A / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode33
Ampicillin
A / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode41
Ampicillin
A / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode49
Ampicillin
A / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode57
Ampicillin
A / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode65
Ampicillin
A / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode73
Ampicillin
A / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode81
Ampicillin
A / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode89
Ampicillin
B / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode2
Ampicillin
B / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode10
Ampicillin
B / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode18
Ampicillin
B / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode26
Ampicillin
B / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode34
Ampicillin
B / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode42
Ampicillin
B / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode50
Ampicillin
B / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode58
Ampicillin
B / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode66
Ampicillin
B / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode74
Ampicillin
B / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode82
Ampicillin
B / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode90
Ampicillin
C / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode3
Ampicillin
C / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode11
Ampicillin
C / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode19
Ampicillin
C / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode27
Ampicillin
C / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode35
Ampicillin
C / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode43
Ampicillin
C / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode51
Ampicillin
C / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode59
Ampicillin
C / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode67
Ampicillin
C / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode75
Ampicillin
C / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode83
Ampicillin
C / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode91
Ampicillin
D / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode4
Ampicillin
D / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode12
Ampicillin
D / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode20
Ampicillin
D / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode28
Ampicillin
D / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode36
Ampicillin
D / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode44
Ampicillin
D / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode52
Ampicillin
D / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode60
Ampicillin
D / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode68
Ampicillin
D / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode76
Ampicillin
D / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode84
Ampicillin
D / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode92
Ampicillin
E / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode5
Ampicillin
E / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode13
Ampicillin
E / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode21
Ampicillin
E / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode29
Ampicillin
E / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode37
Ampicillin
E / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode45
Ampicillin
E / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode53
Ampicillin
E / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode61
Ampicillin
E / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode69
Ampicillin
E / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode77
Ampicillin
E / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode85
Ampicillin
E / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode93
Ampicillin
F / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode6
Ampicillin
F / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode14
Ampicillin
F / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode22
Ampicillin
F / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode30
Ampicillin
F / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode38
Ampicillin
F / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode46
Ampicillin
F / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode54
Ampicillin
F / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode62
Ampicillin
F / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode70
Ampicillin
F / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode78
Ampicillin
F / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode86
Ampicillin
F / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode94
Ampicillin
G / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode7
Ampicillin
G / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode15
Ampicillin
G / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode23
Ampicillin
G / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode31
Ampicillin
G / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode39
Ampicillin
G / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode47
Ampicillin
G / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode55
Ampicillin
G / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode63
Ampicillin
G / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode71
Ampicillin
G / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode79
Ampicillin
G / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode87
Ampicillin
G / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode95
Ampicillin
H / 1 MSCV-6NBC-GFP-PGK-Puro-Barcode8
Ampicillin
H / 2 MSCV-6NBC-GFP-PGK-Puro-Barcode16
Ampicillin
H / 3 MSCV-6NBC-GFP-PGK-Puro-Barcode24
Ampicillin
H / 4 MSCV-6NBC-GFP-PGK-Puro-Barcode32
Ampicillin
H / 5 MSCV-6NBC-GFP-PGK-Puro-Barcode40
Ampicillin
H / 6 MSCV-6NBC-GFP-PGK-Puro-Barcode48
Ampicillin
H / 7 MSCV-6NBC-GFP-PGK-Puro-Barcode56
Ampicillin
H / 8 MSCV-6NBC-GFP-PGK-Puro-Barcode64
Ampicillin
H / 9 MSCV-6NBC-GFP-PGK-Puro-Barcode72
Ampicillin
H / 10 MSCV-6NBC-GFP-PGK-Puro-Barcode80
Ampicillin
H / 11 MSCV-6NBC-GFP-PGK-Puro-Barcode88
Ampicillin
H / 12 MSCV-6NBC-GFP-PGK-Puro-Barcode96
Ampicillin
Data calculated @ 2025-09-05

Kit Plate Map - #1000000097

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