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ClonTracer Barcoding Library
(Pooled Library #67267, #69830)

  • Purpose

    A high-complexity barcode library that “enables the high-resolution tracking of more than 1 million cancer cells under drug treatment”.

    This library is offered in two sizes, depending on the number of cell lines you want to barcode.


Item Catalog # Description Quantity Price (USD)
Pooled Library 67267 2.5µg pooled library; for amplification or to barcode 1 cell line 1 $540 Add to Cart
Pooled Library 69830 7.5µg pooled library; can barcode approximately 3 cell lines 1 $1300 Add to Cart
Available to Academic and Nonprofits Only

Library Shipping

This library will be delivered as DNA in microcentrifuge tubes on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Concentration

Resource Information

Depositor Comments

Current stock of the library can be used for experiments of less than or equal to 1 million total cell infections.

NOTE: Lentiviral vectors can recombine during library amplification, resulting in an ~1kb band containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain lentiviral packaging sequences or the PuroR gene and will not be selected during experiments.

A recombinant band has been detected in the parent stock of ClonTracer. The concentration of DNA on our website reflects the concentration of the unrecombined plasmid pool and can be used for barcoding as described in the associated protocol.

The proportion of the recombinant band in the pool will increase upon subsequent amplifications. Gel purification will eliminate most of this recombinant band (see example gel) and does not alter barcode representation. If the sample is to be used for amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another amplification with the purified sample.

Diagram of how clontracer library works
Diagram of ClonTracer barcoding process
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    ClonTracer library was a gift from Frank Stegmeier (Addgene # )
  • For your References section:

    Studying clonal dynamics in response to cancer therapy using high-complexity barcoding. Bhang HE, Ruddy DA, Krishnamurthy Radhakrishna V, Caushi JX, Zhao R, Hims MM, Singh AP, Kao I, Rakiec D, Shaw P, Balak M, Raza A, Ackley E, Keen N, Schlabach MR, Palmer M, Leary RJ, Chiang DY, Sellers WR, Michor F, Cooke VG, Korn JM, Stegmeier F. Nat Med. 2015 May;21(5):440-8. doi: 10.1038/nm.3841. PubMed.