SPLINTR Pooled Libraries
(Pooled Library #179774, #179775, #179776)
SPLINTR (Single-cell Profiling and LINeage TRacing) is a collection of three high diversity lentiviral barcode libraries for tracing clonal lineages using bulk and single-cell readouts. Each library comprises a distinct semi-random 60 bp barcode sequence cloned into the 3' untranslated region of one of three fluorescent reporter constructs (eGFP, eBFP, mCHERRY). Barcodes are transcribed and can be robustly detected from three prime single cell protocols without PCR enrichment or by bulk PCR from genomic DNA. The provision of three libraries also allows complex experimental in vitro and in vivo competition experiments to be performed.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||179774||SPLINTR GFP v1||1||$300||Pending|
|Pooled Library||179775||SPLINTR mCHERRY v1||1||$300||Pending|
|Pooled Library||179776||SPLINTR BFP v1||1||$300||Pending|
These libraries are delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
Scripts to pre-process SPLINTR barcoding libraries as performed in Fennell & Vassiliadis et al. Nature 2021 can be found at GitHub - DaneVass/SPLINTR preprocessing (Link opens in a new window).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human CRISPR Metabolic Gene Knockout library was a gift from Mark Dawson (Addgene #)
For your References section:Non-genetic determinants of malignant clonal fitness at single-cell resolution. Fennell KA, Vassiliadis D, Lam EYN, Martelotto LG, Balic JJ, Hollizeck S, Weber TS, Semple T, Wang Q, Miles DC, MacPherson L, Chan Y, Guirguis AA, Kats LM, Wong ES, Dawson S, Naik SH, Dawson MA. Nature. 2022 Jan;601(7891):125-131. doi: 10.1038/s41586-021-04206-7. PubMed 34880496