SPLINTR Pooled Libraries
(Pooled Library #179774, #179775, #179776)

• Purpose:

  SPLINTR (Single-cell Profiling and LINeage TRacing) is a collection of three high diversity lentiviral barcode libraries for tracing clonal lineages using bulk and single-cell readouts. Each library comprises a distinct semi-random 60 bp barcode sequence cloned into the 3' untranslated region of one of three fluorescent reporter constructs (eGFP, eBFP, mCHERRY). Barcodes are transcribed and can be robustly detected from three prime single cell protocols without PCR enrichment or by bulk PCR from genomic DNA. The provision of three libraries also allows complex experimental in vitro and in vivo competition experiments to be performed.

• Vector Backbone:

  SPLINTR GFP v1 backbone

  SPLINTR mCHERRY v1 backbone

  SPLINTR BFP v1 backbone

• Depositing Labs: Mark Dawson
• Publication: Fennell et al. Nature. 2022 Jan;601(7891):125-131. doi: 10.1038/s41586-021-04206-7.

Library Details

• Lentiviral Generation: 3rd

Library Shipping

These libraries are delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20 µL
• Concentration: 20 ng/µL

Resource Information

• Protocols:
  • Re-transformation of the SPLINTR Libraries (PDF, 401 KB)
  • SPLINTR Workflow (PDF, 919 KB)
  • Large-scale Lentivirus Production (PDF, 49 KB)
  • Population-based Barcode Amplification Protocol (PDF, 138 KB)
• Depositor Data:
  • SPLINTER GFP v1 barcode abundance (TXT, 16.7 MB)
  • SPLINTER mCherry v1 barcode abundance (TXT, 138.1 MB)
  • SPLINTER BFP v1 barcode abundance (TXT, 75.1 MB)
• Terms and Licenses:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech) (179775)

Depositor Comments

• SPLINTR is a collection of three high diversity lentiviral barcode libraries for tracing clonal lineages using bulk and single-cell readouts.

Scripts to pre-process SPLINTR barcoding libraries as performed in Fennell & Vassiliadis et al. Nature 2021 can be found at GitHub - DaneVass/SPLINTR preprocessing .