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sgRNA libraries for Pseudomonas aeruginosa PA14
(Pooled Library #234855, #1000000254)

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  • Purpose

    A comprehensive CRISPRi library has been designed for inducible, genome-wide gene repression in Pseudomonas aeruginosa PA14. This library consists of two subsets: Set 1, containing 5,981 sgRNAs targeting distinct genes, and Set 2, comprising 5,971 sgRNAs targeting the same genes as Set 1 but designed for different PAM regions. The sgRNAs in Set 1 and Set 2 complement each other, offering a robust and versatile approach to gene targeting.

    The library has been meticulously engineered for efficient transformation into E. coli strains such as S17 or other E. coli strains carrying the lambda pir gene, which is necessary for R6K ori replication. This system enables the construction of a tetracycline-inducible CRISPRi platform, facilitating precise and controlled gene repression studies.

  • Vector Backbone

    pCRISPRi-ccdB (Plasmid #226432) - expresses dCas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 234855 Set 1, one tube, contains sgRNA library for Pseudomonas aeruginosa PA14 1 $380 Add to Cart
Pooled Library 1000000254 Two tubes, each with a half-library (Set 1 and 2)
  • #234855 (Set 1)
  • #234856 (Set 2)
1 $540 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Pseudomonas aeruginosa
  • Genes targeted
    • Set 1: 5,981
    • Set 2: 5,971
  • gRNAs
    1 sgRNA per gene in each set

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼25 µL
  • Concentration
    100 ng/µL

Resource Information

Depositor Comments

Set 1 is a primary set of 5,981 sgRNAs with high specificity targeting all operons and genes in the P. aeruginosa PA14 genome. Set 2 is a secondary set of 5,971 sgRNAs providing alternative guides to ensure full genome coverage in P. aeruginosa PA14.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sgRNA libraries Set 1 for Pseudomonas aeruginosa PA14 was a gift from Xue Liu (Addgene #234855 ; http://n2t.net/addgene:234855 ; RRID:Addgene_234855)
    sgRNA libraries Set 1 and 2 for Pseudomonas aeruginosa PA14 was a gift from Xue Liu (Addgene #1000000254)

  • For your References section:

    Genome-wide CRISPRi-seq Identified Ferredoxin-NADP Reductase FprB as a Synergistic Target for Gallium Therapy in Pseudomonas aeruginosa. Zhang Y, Zhang T, Xiao X, Kawalek A, Ou J, Ren A, Sun W, de Bakker V, Liu Y, Li Y, Yang L, Ye L, Jia N, Veening JW, Wang Y, Liu X. bioRxiv 2024.09.01.610675. doi: 10.1101/2024.09.01.610675.
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