- Purpose
Version 2 of the human CRISPR/Cas9 SAM (Synergistic Activation Mediator) pooled library. This library uses an engineered protein complex for the transcriptional activation of endogenous genes.
For SAM gain-of-function screening, this gRNA library has to be combined with 1 additional SAM construct, MS2-P65-HSF1 (Addgene plasmid #89308), which is provided with the library.
- Vector BackbonelentiSAMv2- this backbone contains dCas9-VP64-Blast and sgRNA(MS2)
Note: Libraries ordered prior to 4/3/2017 were shipped with Addgene plasmid #61426 rather than #89308
- Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 1000000078 | gRNA pooled library in lentiSAMv2 + MS2-P65-HSF1 activator plasmid | 1 | $ 640 | Add to Cart |
Library Details
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SpeciesHuman
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Genes targeted23,430
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gRNAs70,290
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Lentiviral Generation3rd
Library Shipping
Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration50 ng/µL
You will also receive a bacterial stab of the MS2-P65-HSF1 plasmid (Addgene #89308). Please do NOT order this plasmid in addition to this library.
Resource Information
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Protocols
Find detailed instructions on using this library in the protocol paper from Joung, et al. -
Depositor Data
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Terms and Licenses
Depositor Comments
The SAM library consists of 3 unique sgRNAs targeting the RefSeq coding isoforms in the proximal promoter (>90% of sgRNAs are targeted to the first 200bp upstream of the transcription start site of their target).

For additional information, protocols and an activator sgRNA design tool, visit the Zhang Lab SAM website:
http://sam.genome-engineering.org/
The publication describing this pooled library is available in Nature Protocols.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
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Example for your Materials & Methods section:
Human CRISPR 2-plasmid activation pooled library (SAM) was a gift from Feng Zhang (Addgene # 1000000078) For your References section:
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, & Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed 28333914