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Addgene

Human CRISPR Activation Library (SAM - 2 plasmid system)
(Pooled Library #1000000078)

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  • Purpose

    Version 2 of the human CRISPR/Cas9 SAM (Synergistic Activation Mediator) pooled library. This library uses an engineered protein complex for the transcriptional activation of endogenous genes.

    For SAM gain-of-function screening, this gRNA library has to be combined with 1 additional SAM construct, MS2-P65-HSF1 (Addgene plasmid #89308), which is provided with the library.

    • Note: Libraries ordered prior to 4/3/2017 were shipped with Addgene plasmid #61426 rather than #89308

  • Vector Backbone
    lentiSAMv2- this backbone contains dCas9-VP64-Blast and sgRNA(MS2)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000078 gRNA pooled library in lentiSAMv2 + MS2-P65-HSF1 activator plasmid 1 $ 640 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    23,430
  • gRNAs
    70,290
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

You will also receive a bacterial stab of the MS2-P65-HSF1 plasmid (Addgene #89308). Please do NOT order this plasmid in addition to this library.

Resource Information

Depositor Comments

The SAM library consists of 3 unique sgRNAs targeting the RefSeq coding isoforms in the proximal promoter (>90% of sgRNAs are targeted to the first 200bp upstream of the transcription start site of their target).

Diagram of lentiSAMv2 backbone
Diagram of lentiSAMv2 backbone

For additional information, protocols and an activator sgRNA design tool, visit the Zhang Lab SAM website:
http://sam.genome-engineering.org/

The publication describing this pooled library is available in Nature Protocols.

How to cite this pooled library ( Back to top)

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Human CRISPR 2-plasmid activation pooled library (SAM) was a gift from Feng Zhang (Addgene # 1000000078)

  • For your References section:

    Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, & Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed 28333914