Mouse CRISPR Activation Library (SAM - 3 plasmid system)
(Pooled Library #1000000075)
The mouse CRISPR/Cas9 Synergistic Activation Mediator (SAM) pooled library uses an engineered protein complex for the transcriptional activation of endogenous genes.
This library consists of three components which are all provided :
- A nucleolytically inactive Cas9-VP64 fusion (Addgene plasmid # 61425)
- A gRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2 (present in the libraries)
- The MS2-P65-HSF1 plasmid which expresses the activation helper protein (Addgene plasmid #89308)
Note: Libraries ordered prior to 4/3/2017 were shipped with Addgene plasmid #61426 rather than #89308
- Vector Backbone
- Depositing Labs
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Mouse CRISPR 3-plasmid activation pooled library (SAM) was a gift from Feng Zhang (Addgene #1000000075)
For your References section:Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, & Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed 28333914