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Plasmids

  1. pGTvL1-SGC (Plasmid #39188) Empty backbone
    Purpose SGC Empty backbone for bacterial expression
    Insert: none
    Tags: GST TEV
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Nicola Burgess-Brown
  2. pTvHR21-SGC (Plasmid #39186) Empty backbone
    Purpose SGC Empty backbone for bacterial expression
    Insert: none
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Nicola Burgess-Brown
  3. pLIC-SGC1 (Plasmid #39187) Empty backbone
    Purpose SGC Empty backbone for bacterial expression
    Insert: none
    Tags: His Tev
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Nicola Burgess-Brown
  4. pBEN1-SGC (Plasmid #39127) Empty backbone
    Purpose SGC Empty backbone for bacterial expression
    Insert: none
    Tags: Strep tag and SET1
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Nicola Burgess-Brown
  5. SGCD-His-bio (Plasmid #52333)
    Purpose Expresses full-length Delta-sarcoglycan ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Insert: SGCD ( SGCD Human )
    Tags: rat CD4 d3+4, enzymatic bioti...
    Expression: Mammalian
    Mutation: None
    Use:
    Deposited by: Gavin Wright
  6. pAG180 SGCD 3'UTR mut (Plasmid #12038)
    Insert: N13 3'UTR mut ( SGCD Human )
    Tags: None
    Expression: Mammalian
    Mutation: Mutations in microRNA recogni...
    Use: Luciferase
    Deposited by: David Bartel
  7. pAG148 SGCD 3'UTR (Plasmid #12006)
    Insert: N13 3'UTR ( SGCD Human )
    Tags: None
    Expression: Mammalian
    Mutation: None
    Use: Luciferase
    Deposited by: David Bartel
  8. pAC1454_pX-sgCentromere-25xPBSc (Plasmid #73696)
    Purpose Vector expression sgCentromere-20xPBSc
    Insert: sgCentromere-20xPBSc
    Tags: None
    Expression: Mammalian
    Mutation: None
    Use: CRISPR
    Deposited by: Albert Cheng
  9. SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE (Plasmid #87919)
    Purpose HIV-1 SIN lentiviral transfer vector
    Insert: sgHTT1, shGFP2, sgCas9, cherry Synthetic
    Tags: None
    Expression:
    Mutation: None
    Use: Lentiviral
    Deposited by: Nicole Déglon
  10. pENTR221-H1-sgGFP1-7SK-sgCas9 (Plasmid #87908)
    Purpose multiple sgRNA
    Insert: sgGFP1, sgCas9 Synthetic
    Tags: None
    Expression:
    Mutation: None
    Use: Gateway entry vector
    Deposited by: Nicole Déglon
  11. SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE (Plasmid #87912)
    Purpose Kamicas9 lentiviral vector
    Insert: shHTT1, sgCas9, cherry
    Tags: None
    Expression:
    Mutation: None
    Use: Lentiviral
    Deposited by: Nicole Déglon
  12. pU6-sgCXCR4-2 (Plasmid #46917)
    Purpose Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene
    Insert: sgCXCR4 -2 ( CXCR4 Human )
    Insert: Puromycin resistance and mCherry
    Tags: None
    Expression: Mammalian
    Mutation: None
    Use: Lentiviral, CRISPR
    Deposited by: Stanley Qi
  13. pETDuet-msGC-NT21 (Plasmid #78102)
    Purpose expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Insert: fragment of alfa subunit of sGC manduca sexta (tobacco hornworm)
    Insert: fragment of beta subunit of sGC manduca sexta (tobacco hornworm)
    Tags: His-tag
    Expression: Bacterial
    Mutation: fragment 272-450 of the alfa ...
    Use:
    Deposited by: William Montfort
  14. pETDuet-msGC-NT2 (Plasmid #74763)
    Purpose expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Insert: fragment of alfa subunit of sGC manduca sexta (tobacco hornworm)
    Insert: fragment of beta subunit of sGC manduca sexta (tobacco hornworm)
    Tags: His-tag
    Expression: Bacterial
    Mutation: fragment 49-471 of the alfa s...
    Use:
    Deposited by: William Montfort
  15. RGS16 (Plasmid #39140)
    Purpose Bacterial expression for structure determination; may not be full ORF
    Insert: RGS16 ( RGS16 Human )
    Tags: His Tev
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Nicola Burgess-Brown
  16. RGS10 (Plasmid #39138)
    Purpose Bacterial expression for structure determination; may not be full ORF
    Insert: RGS10 ( RGS10 Human )
    Tags: His Tev
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Nicola Burgess-Brown
  17. RND1 (Plasmid #39145)
    Purpose Bacterial expression for structure determination; may not be full ORF
    Insert: RND1 ( RND1 Human )
    Tags: His Tev
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Nicola Burgess-Brown
  18. pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP (Plasmid #84253)
    Purpose Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker
    Insert: Sp sgCD95-3 Human, Synthetic; S. pyogenes
    Tags: None
    Expression: Mammalian
    Mutation: None
    Use: Lentiviral, CRISPR
    Deposited by: Stanley Qi
  19. DHRS11 (Plasmid #39157)
    Purpose Bacterial expression for structure determination; may not be full ORF
    Insert: DHRS11 ( DHRS11 Human )
    Tags: His Tev
    Expression: Bacterial
    Mutation: G99L100 -> AHM; codon-optimized
    Use:
    Deposited by: Nicola Burgess-Brown
  20. GMDS (Plasmid #39152)
    Purpose Bacterial expression for structure determination; may not be full ORF
    Insert: GMDS ( GMDS Human )
    Tags: His Tev
    Expression: Bacterial
    Mutation: codon-optimized
    Use:
    Deposited by: Nicola Burgess-Brown
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