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Plasmid#221662PurposeFor Agrobacterium-mediated transformation. Plastid(outer envelope membrane)-localized mCherry-UnaG.DepositorInsertmCherry-UnaG flanked with an N-terminal sequence of Arabidopsis OUTRE ENVELOPE PROTEIN7
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDNgp
Plasmid#162453PurposePositive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.DepositorInsertAga2p-2Glinker-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsHA nad myc and eUnaG2 fluorescent proteinExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDNg
Plasmid#162452PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHa and myc and eUnaG2 fluorescent proteinExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDNp
Plasmid#162451PurposePositive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.DepositorInsertAga2p-eUnaG2
UseShuttle vector bacteria/yeastTagsHA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDC2
Plasmid#162459PurposeYeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only