Skip to main content
Addgene
Showing: 1 - 9 of 9 results
  1. pGWB602-oeUnaG

    Plasmid
    #221662
    Purpose
    For Agrobacterium-mediated transformation. Plastid(outer envelope membrane)-localized mCherry-UnaG.
    Depositor
    Insert
    mCherry-UnaG flanked with an N-terminal sequence of Arabidopsis OUTRE ENVELOPE PROTEIN7
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pJYDNgp

    Plasmid
    #162453
    Purpose
    Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.
    Depositor
    Insert
    Aga2p-2Glinker-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA nad myc and eUnaG2 fluorescent protein
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pJYDNg

    Plasmid
    #162452
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.
    Depositor
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Ha and myc and eUnaG2 fluorescent protein
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pJYDC1

    Plasmid
    #162458
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus
    Depositor
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA tag, HDEL sequence, eUnaG2 fluorescent protein…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pJYDN

    Plasmid
    #162450
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.
    Depositor
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pJYDN3

    Plasmid
    #162456
    Purpose
    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.
    Depositor
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pJYDC2

    Plasmid
    #162459
    Purpose
    Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.
    Depositor
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 9 of 9 results