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49 Results for:  streptococcus -(crispr) -(cas9)

Your Selections:

Plasmids, Strains, and Pooled Libraries 1–20 of 49

  1. Plasmid
    #108550
    Depositor
    Purpose
    Bacterial Target-AID vector with sgRNA
    Insert
    dCas9-PmCDA1 Synthetic; Streptococcus pyogenes
    Insert
    Streptococcus pyogenes sgRNA Synthetic
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    D10A and H840A for SpCas9
  2. Plasmid
    #83372
    Depositor
    Purpose
    Expresses SPN and IFS complex in E. coli cell
    Insert
    SPN Streptococcus pyogenes serotype M3
    Insert
    IFS Streptococcus pyogenes serotype M3
    Use
    Tags
    Hexahistidine tag
    Expression
    Bacterial
    Mutation
    None
  3. Plasmid
    #108552
    Depositor
    Purpose
    Bacterial sgRNA expression vector
    Insert
    2x Streptococcus pyogenes sgRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  4. Plasmid
    #58333
    Depositor
    Purpose
    Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein
    Insert
    PepTSt Streptococcus thermophilus
    Use
    Tags
    GFP-His
    Expression
    Bacterial
    Mutation
    None
  5. Plasmid
    #64987
    Depositor
    Purpose
    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli
    Insert
    S. pyogenes sortase A Streptococcus pyogenes M1 GAS
    Use
    Tags
    chitin-binding protein, His6 ...
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-81
  6. Plasmid
    #119951
    Depositor
    Purpose
    Expresses recombinant MVA pathway in Escherichia coli
    Insert
    mvaE Enterococcus faecalis
    Insert
    mvaS Enterococcus faecalis
    Insert
    mvaK1 Streptococcus pneumoniae
    Insert
    mvaK2 Streptococcus pneumoniae
    Insert
    mvaD Streptococcus pneumoniae
    Insert
    idi Escherichia coli
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    A110G
  7. Plasmid
    #121051
    Depositor
    Purpose
    Expresses recombinant MVA pathway in Escherichia coli
    Insert
    mvaE Enterococcus faecalis
    Insert
    mvaS Enterococcus faecalis
    Insert
    mvaK1 Streptococcus pneumoniae
    Insert
    mvaK2 Streptococcus pneumoniae
    Insert
    mvaD Streptococcus pneumoniae
    Insert
    idi Escherichia coli
    Use
    Tags
    None
    Expression
    Mutation
    None
  8. Plasmid
    #35044
    Depositor
    Insert
    SpyCatcher Streptococcus pyogenes
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Deletion of C-terminal till I...
  9. Plasmid
    #108551
    Depositor
    Purpose
    Bacterial Target-AID UGI-LVA vector
    Insert
    dCas9-PmCDA1-UGI-LVA Synthetic; Streptococcus pyogenes
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    D10A and H840A for SpCas9
  10. Plasmid
    #123612
    Depositor
    Purpose
    CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.
    Insert
    BE3-P2A-EGFP Rat, Synthetic; Streptococcus pyogenes, Bacillus subtilis bacteriophage PBS1
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
  11. Plasmid
    #108549
    Depositor
    Purpose
    Bacterial Target-AID vector
    Insert
    dCas9-PmCDA1 Synthetic; Streptococcus pyogenes
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    D10A and H840A for SpCas9
  12. Plasmid
    #50030
    Depositor
    Purpose
    Expression of cytosolic S. pyogenes SrtA in mammalian cells
    Insert
    SrtA(strep) Streptococcus pyogenes
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    None
  13. Plasmid
    #123613
    Depositor
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).
    Insert
    BE3(E63Q)-P2A-EGFP Rat, Synthetic; Streptococcus pyogenes, Bacillus subtilis bacteriophage PBS1
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    E63Q in rAPOBEC1, D10A in SpCas9
  14. Plasmid
    #69105
    Depositor
    Purpose
    Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.
    Insert
    mKate2 Synthetic
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  15. Plasmid
    #60576
    Depositor
    Purpose
    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
    Insert
    Integrase_4 Streptococcus equi subsp. equi 4047
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli
  16. Plasmid
    #79053
    Depositor
    Purpose
    Expresses maltose binding protein (MBP) SpyCatcher fusion
    Insert
    MBP-SpyCatcher Streptococcus pyogenes
    Use
    Tags
    Maltose binding protein, 6xHis
    Expression
    Bacterial
    Mutation
    Derived from pDEST14-SpyCatch...
  17. Plasmid
    #86837
    Depositor
    Purpose
    bacterial expression of AcrIIA3
    Insert
    acrIIA3 Streptococcus pyogenes
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  18. Plasmid
    #78492
    Depositor
    Purpose
    Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.
    Insert
    tracrRNA E. coli
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  19. Plasmid
    #127413
    Depositor
    Purpose
    Expresses DpnII restriction endonuclease in bacteria
    Insert
    DpnII streptococcus pneumoniae
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  20. Plasmid
    #123614
    Depositor
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).
    Insert
    BE3(R33A)-P2A-EGFP Rat, Synthetic; Streptococcus pyogenes, Bacillus subtilis bacteriophage PBS1
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, D10A in SpCas9
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