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68 Results for:  streptococcus -(crispr) -(cas9)

Your Selections:

Plasmids, Strains, and Pooled Libraries 1–20 of 68

  1. Plasmid
    #128498
    Depositor
    Purpose
    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.
    Insert
    GRAB (Streptococcus pyogenes serotype M1) ( grab Streptococcus pyogenes serotype M1 )
    Use
    Tags
    His tag (6x)
    Expression
    Bacterial
    Mutation
    None
  2. Plasmid
    #108550
    Depositor
    Purpose
    Bacterial Target-AID vector with sgRNA
    Insert
    dCas9-PmCDA1 Synthetic; Streptococcus pyogenes
    Insert
    Streptococcus pyogenes sgRNA Synthetic
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    D10A and H840A for SpCas9
  3. Plasmid
    #83372
    Depositor
    Purpose
    Expresses SPN and IFS complex in E. coli cell
    Insert
    SPN Streptococcus pyogenes serotype M3
    Insert
    IFS Streptococcus pyogenes serotype M3
    Use
    Tags
    Hexahistidine tag
    Expression
    Bacterial
    Mutation
    None
  4. Plasmid
    #108552
    Depositor
    Purpose
    Bacterial sgRNA expression vector
    Insert
    2x Streptococcus pyogenes sgRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  5. Plasmid
    #58333
    Depositor
    Purpose
    Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein
    Insert
    PepTSt Streptococcus thermophilus
    Use
    Tags
    GFP-His
    Expression
    Bacterial
    Mutation
    None
  6. Plasmid
    #64987
    Depositor
    Purpose
    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli
    Insert
    S. pyogenes sortase A Streptococcus pyogenes M1 GAS
    Use
    Tags
    chitin-binding protein, His6 ...
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-81
  7. Plasmid
    #119951
    Depositor
    Purpose
    Expresses recombinant MVA pathway in Escherichia coli
    Insert
    mvaE Enterococcus faecalis
    Insert
    mvaS Enterococcus faecalis
    Insert
    mvaK1 Streptococcus pneumoniae
    Insert
    mvaK2 Streptococcus pneumoniae
    Insert
    mvaD Streptococcus pneumoniae
    Insert
    idi Escherichia coli
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    A110G
  8. Plasmid
    #121051
    Depositor
    Purpose
    Expresses recombinant MVA pathway in Escherichia coli
    Insert
    mvaE Enterococcus faecalis
    Insert
    mvaS Enterococcus faecalis
    Insert
    mvaK1 Streptococcus pneumoniae
    Insert
    mvaK2 Streptococcus pneumoniae
    Insert
    mvaD Streptococcus pneumoniae
    Insert
    idi Escherichia coli
    Use
    Tags
    None
    Expression
    Mutation
    None
  9. Plasmid
    #128738
    Depositor
    Purpose
    Expresses GB1 fused to GyrA intein at the C-terminal
    Insert
    GB1 Streptococcus sp. group G
    Use
    Tags
    GyrA
    Expression
    Bacterial
    Mutation
    None
  10. Plasmid
    #35044
    Depositor
    Insert
    SpyCatcher Streptococcus pyogenes
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Deletion of C-terminal till I...
  11. Plasmid
    #108551
    Depositor
    Purpose
    Bacterial Target-AID UGI-LVA vector
    Insert
    dCas9-PmCDA1-UGI-LVA Synthetic; Streptococcus pyogenes
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    D10A and H840A for SpCas9
  12. Plasmid
    #123612
    Depositor
    Purpose
    CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.
    Insert
    BE3-P2A-EGFP Rat, Synthetic; Streptococcus pyogenes, Bacillus subtilis bacteriophage PBS1
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
  13. Plasmid
    #108549
    Depositor
    Purpose
    Bacterial Target-AID vector
    Insert
    dCas9-PmCDA1 Synthetic; Streptococcus pyogenes
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    D10A and H840A for SpCas9
  14. Plasmid
    #50030
    Depositor
    Purpose
    Expression of cytosolic S. pyogenes SrtA in mammalian cells
    Insert
    SrtA(strep) Streptococcus pyogenes
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    None
  15. Plasmid
    #131312
    Depositor
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS E.coli, Streptococcus pyogenes
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  16. Plasmid
    #131300
    Depositor
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP Streptococcus pyogenes, Bacillus subtilis bacteriophage PBS1, SV40, Petromyzon marinus
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  17. pLZts Empty backbone
    Plasmid
    #128799
    Depositor
    Purpose
    Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus
    Use
    Bacterial mutagenesis
    Tags
    None
    Expression
    Mutation
    None
  18. pASR130 Empty backbone
    Plasmid
    #122638
    Depositor
    Purpose
    BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  19. pPEPY Empty backbone
    Plasmid
    #122633
    Depositor
    Purpose
    BglFusion compatible integration vector for Streptococcus pneumoniae
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  20. Plasmid
    #123613
    Depositor
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).
    Insert
    BE3(E63Q)-P2A-EGFP Rat, Synthetic; Streptococcus pyogenes, Bacillus subtilis bacteriophage PBS1
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    E63Q in rAPOBEC1, D10A in SpCas9
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