We narrowed to 184 results for: primer

1. PrimeRoot.v2C-Cre

   Plasmid

   #201739

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,Cre
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PrimeRoot.v2C-FLP

   Plasmid

   #201740

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,FLP
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PrimeRoot.v2C-Cre

   Plasmid

   #201739

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,Cre
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PrimeRoot.v2C-FLP

   Plasmid

   #201740

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,FLP
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC6

   Plasmid

   #49793

   Purpose

   E. coli cloning vector

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1982 Oct;19(3):259-68.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pUC6

   Plasmid

   #49793

   Purpose

   E. coli cloning vector

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1982 Oct;19(3):259-68.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUC119 T7 hTRmin

   Plasmid

   #127858

   Purpose

   T7 RNA Pol for in vitro transcription

   Depositor

   Kathleen Collins

   Article

   Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

   Insert

   hTR (TERC Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC119 T7 hTRmin

   Plasmid

   #127858

   Purpose

   T7 RNA Pol for in vitro transcription

   Depositor

   Kathleen Collins

   Article

   Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

   Insert

   hTR (TERC Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-HA-Ebp1

   Plasmid

   #67792

   Purpose

   Mammalian expression of Ebp1 tagged with HA

   Depositor

   Stephen Goff

   Article

   Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

   Insert

   Ebp1 (PA2G4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pTBY1-T4gp61/41 fusion

    Plasmid

    #228210

    Purpose

    Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

    Depositor

    Stephen Benkovic

    Article

    Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

    Insert

    61/41
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-HA-Ebp1

    Plasmid

    #67792

    Purpose

    Mammalian expression of Ebp1 tagged with HA

    Depositor

    Stephen Goff

    Article

    Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

    Insert

    Ebp1 (PA2G4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTBY1-T4gp61/41 fusion

    Plasmid

    #228210

    Purpose

    Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

    Depositor

    Stephen Benkovic

    Article

    Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

    Insert

    61/41
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p_m16S

    Plasmid

    #160549

    Purpose

    Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline

    Depositor

    Denis Gerlier

    Article

    Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do

    Insert

    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-Impact-T4gp61(I342G)

    Plasmid

    #228208

    Purpose

    Expression in BL21(DE3) of T4 primase I342G

    Depositor

    Stephen Benkovic

    Article

    Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

    Insert

    61 (61 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated isoleucine 342 to glycine (I342G), improv…

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p_m16S

    Plasmid

    #160549

    Purpose

    Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline

    Depositor

    Denis Gerlier

    Article

    Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do

    Insert

    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-Impact-T4gp61(I342G)

    Plasmid

    #228208

    Purpose

    Expression in BL21(DE3) of T4 primase I342G

    Depositor

    Stephen Benkovic

    Article

    Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

    Insert

    61 (61 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated isoleucine 342 to glycine (I342G), improv…

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGLACTE-PseudoHT51

    Plasmid

    #99257

    Purpose

    Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

    Depositor

    Karen Usdin

    Article

    Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

    Insert

    FMR1 (FMR1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mutation

    Contains synthetic, non-human, primer sequence do…

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGLACTE-PseudoHT51

    Plasmid

    #99257

    Purpose

    Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

    Depositor

    Karen Usdin

    Article

    Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

    Insert

    FMR1 (FMR1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mutation

    Contains synthetic, non-human, primer sequence do…

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Use

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

    Plasmid

    #70195

    Purpose

    Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    tetR-deGFP fusion
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge