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Plasmid#173840PurposeA knock-out vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-puro-dHbegf
Plasmid#173841PurposeA knockout vector for the dog Hbegf.DepositorInsertA gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-hyg2-dEreg
Plasmid#173843PurposeA knockout vector for the dog Ereg.DepositorInsertA gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisHbegf
Plasmid#173846PurposeEncoding dog Hbegf.DepositorInsertA cDNA of canis Hbegf.
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisTgfa
Plasmid#173847PurposeEncoding dog Tgfa.DepositorInsertA cDNA of canis Tgfa.
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisEreg
Plasmid#173848PurposeEncoding dog Ereg.DepositorInsertA cDNA of canis Tgfa.
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N1-HDAC6.delta Buz
Plasmid#36190DepositorInsertHDAC6..delta Buz (HDAC6 Human)
UseTagsEGFPExpressionMammalianMutationDeletion of the C-terminal ub binding domain (BUZ…PromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-ErbB1(DEL1011)
Plasmid#197366PurposeEncoding human ErbB1 mutant.DepositorInsertEGFR mutant. (EGFR Human)
UseTagsExpressionMammalianMutationdeleted amino acids 1011-1186PromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcS-RDG-C1C2
Plasmid#200163PurposeThis plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsRDG-C1C2
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-ErbB1(F992)
Plasmid#197365PurposeEncoding human ErbB1 mutant.DepositorInsertEGFR mutant (EGFR Human)
UseTagsExpressionMammalianMutationTyrosine 992 to PhenylalaninePromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEGF-5’UTR
Plasmid#177260PurposeA knockout vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-loxP-canisNrg1
Plasmid#173849PurposeEncoding dog Nrg1 before Cre-mediated recombination.DepositorInsertA cDNA of canis Nrg1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEgf
Plasmid#173700PurposeA knock-out vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-neo-dTgfa
Plasmid#173842PurposeA knockout vector for the dog Tgfa.DepositorInsertA gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisEgf
Plasmid#173845PurposeEncoding dog Egf.DepositorInsertA cDNA of canis EGF.
UseTagsExpressionMammalianMutationR1127Q- please see dep. commentsPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
R725-X09-635: His6-tev-Hs.SPRED1(13-125)
Plasmid#159581PurposeBaculovirus protein expression of His6-tev-Hs.SPRED1(13-125)DepositorInsertHis6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKUNOC-ErbB1(M721)-mScarlet
Plasmid#197364PurposeEncoding human ErbB1 mutant tagged with mScarlet.DepositorInsertEGFR mutant (EGFR Human)
UseTagsLysine 721 to Methionine and mScarletExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorInsertHis6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorInsertHis6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
RCAS-sgATG7
Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorInsertAutophagy Related 7 (Atg7 Mouse)
UseRetroviralTagsExpressionMutationPromoterU6Available sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dL5
Plasmid#73214PurposeBacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsertEGFR (EGFR Human, Schistosoma japonicum)
UseTags10XHis-GST-HRVExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX303-PCDH7-a
Plasmid#108538PurposeExpresses human PCDH7 transcript variant a in mammalian cellsDepositorInsertPCDH7 transcript variant a (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-21 Reporter
Plasmid#71870PurposeMammalian expression vector for the analysis of miR-21 activityDepositorInsertReverse complementary sequence of miR-21
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2/GD-IRES-GFP-CTNNB1
Plasmid#192868PurposeCarries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.DepositorInsertsluc+
EGFP
CTNNB1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIR-31-Reporter
Plasmid#71871PurposeMammalian expression vector for the analysis of miR-31 activityDepositorInsertReverse complementary sequence of miR-31
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-192-Reporter
Plasmid#71872PurposeMammalian expression vector for the analysis of miR-19α activityDepositorInsertReverse complementary sequence of miR-192
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-193a-5p-Reporter
Plasmid#71873PurposeMammalian expression vector for the analysis of miR-193a-5p activityDepositorInsertReverse complementary sequence of miR-193a-5p
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX303-PCDH7-d
Plasmid#108541PurposeExpresses human PCDH7 transcript variant d in mammalian cellsDepositorInsertPCDH7 transcript variant d (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only