We narrowed to 20,725 results for: Kin

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Desmoplakin II no force control (FL-based)

Plasmid

#118724

Purpose

The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry

Expression

Mammalian

Mutation

truncation after aa1353

Promoter

CMV

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-HA-mCherry-T2A-Tmsb10

Plasmid

#191137

Purpose

Knock in HA-T2A-mCherry into the mouse Tmsb10 gene locus

Depositor

Ken-ichirou Morohashi

Article

Inoue et al Commun Biol. 2022 Sep 15;5(1):974. doi:

Insert

HA-mCherry-T2A-Tmsb10

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Sc FLAG-kin-mec3-pRSFDuet

Plasmid

#240741

Purpose

Over-express Sc FLAG-PKA kinase motif-mec3 in E. coli

Depositor

Michael O'Donnell

Insert

FLAG-kin-mec3 (MEC3 Budding Yeast)

Tags

3X FLAG followed by PKA kinase motif

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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ABL1-N-HiBiT kinase domain_v1

Plasmid

#207121

Purpose

Bacterially expressed HiBiT-ABL1 kinase domain (D233-S500) for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Human Abelson Kinase Domain N-terminal HiBiT, N-terminal 10X His-tag (ABL1 Human)

Tags

HiBiT Tag and His-tag with thrombin site

Expression

Bacterial

Mutation

Codon optmization for expression in BL21DE3

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

Plasmid

#233368

Purpose

hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40

Plasmid

#233367

Purpose

hSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA_T/A-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP

Plasmid

#233356

Purpose

CMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_off-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP

Plasmid

#233354

Purpose

CMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP

Plasmid

#233360

Purpose

CMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_JNK_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP

Plasmid

#233358

Purpose

CMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKC_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

Plasmid

#233355

Purpose

CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP

Plasmid

#233362

Purpose

CMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_AMPK_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP

Plasmid

#233369

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Use

Lentiviral

Tags

NES and mEGFP

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP

Plasmid

#233370

Purpose

CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Use

Lentiviral

Tags

NES and mEGFP

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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NOLC1 nicking guide pDY2251

Plasmid

#219861

Purpose

Nicking sgRNA for NOLC1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 nicking sgRNA (NOLC1 Human)

Use

CRISPR

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin Ubl P37L

Plasmid

#227366

Purpose

For bacterial expression of mutated parkin Ubl.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin 1-76 P37L (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3346 PB-nicking guide-blast

Plasmid

#226665

Purpose

PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

protective nicking sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin(C431N)-A92mKO2

Plasmid

#213545

Purpose

Transient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Expression

Mammalian

Mutation

C431N mutation in Parkin

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A-Kina

Plasmid

#202745

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-hMeikin(8A mut)

Plasmid

#174707

Purpose

Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Tags

EGFP

Expression

Mammalian

Mutation

S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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