We narrowed to 419 results for: Pef1

1. PAM10

   Plasmid

   #198743

   Purpose

   pEF1-GFP, Geneticin resistance

   Depositor

   Chantal Abergel

   Article

   Philippe et al Nat Protoc. 2023 Nov 14. doi: 10.1038/s4

   Insert

   GFP
   

   Use

   Acanthamoeba expression

   Tags

   Expression

   Mutation

   Promoter

   pEF1

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEFX-AP

   Plasmid

   #31234

   Depositor

   Cliff Ragsdale

   Article

   Agarwala et al Science. 2001 Mar 16. 291(5511):2147-50.

   Insert

   AP (ALPP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pEF‐PIK3IP1

   Plasmid

   #49214

   Purpose

   expresses PIK3IP1 in mammalian cells

   Depositor

   Lawrence Kane

   Article

   DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

   Insert

   PIK3IP1 (Pik3ip1 Mouse)
   

   Use

   Tags

   6X-his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. Integrin alpha5-AHCys

   Plasmid

   #27313

   Depositor

   Timothy Springer

   Article

   Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

   Insert

   integrin alpha 5 (ITGA5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   integrin alpha5 (1-954) + AHCys

   Promoter

   Available since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

5. Integrin alpha5(1-623)-AHCys

   Plasmid

   #27301

   Depositor

   Timothy Springer

   Article

   Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

   Insert

   integrin alpha 5 (ITGA5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   alpha 5(1-623)+GG+TEV+GGEN+AHCys

   Promoter

   Available since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pEFX-AP

   Plasmid

   #31234

   Depositor

   Cliff Ragsdale

   Article

   Agarwala et al Science. 2001 Mar 16. 291(5511):2147-50.

   Insert

   AP (ALPP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pEF-APEX2-NLS

   Plasmid

   #140281

   Purpose

   Expression of APEX2-NLS

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   APEX2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF‐PIK3IP1

   Plasmid

   #49214

   Purpose

   expresses PIK3IP1 in mammalian cells

   Depositor

   Lawrence Kane

   Article

   DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

   Insert

   PIK3IP1 (Pik3ip1 Mouse)
   

   Use

   Tags

   6X-his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

9. PM PKA Reporter

   Plasmid

   #104551

   Purpose

   Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Lyn-mTurquoise2-VASP 148 - 164
   

   Use

   Tags

   mTurquoise 2, HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Integrin alpha5-AHCys

    Plasmid

    #27313

    Depositor

    Timothy Springer

    Article

    Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

    Insert

    integrin alpha 5 (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    integrin alpha5 (1-954) + AHCys

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

11. OMM PKA Reporter

    Plasmid

    #104550

    Purpose

    Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Tom20 - mTurquoise2 - VASP 148-164
    

    Use

    Tags

    mTurqouise2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrin alpha5(1-623)-AHCys

    Plasmid

    #27301

    Depositor

    Timothy Springer

    Article

    Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

    Insert

    integrin alpha 5 (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alpha 5(1-623)+GG+TEV+GGEN+AHCys

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pHPHS800

    Plasmid

    #228240

    Purpose

    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

    Insert

    Cas9
    

    Use

    Tags

    Hygromycin phosphotransferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPRa_all-in-one_Bip gRNA1

    Plasmid

    #183696

    Purpose

    Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    gRNA targeting chinese hamster Bip (Hspa5)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDD2604

    Plasmid

    #175088

    Purpose

    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S644A

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD2601

    Plasmid

    #175085

    Purpose

    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S47A, S65A, T75A

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDD2602

    Plasmid

    #175086

    Purpose

    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T424A, T438A

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDD2603

    Plasmid

    #175087

    Purpose

    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T504A, T508A

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

    Plasmid

    #160046

    Purpose

    For expressing the full paradoxical circuit

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Inserts

    osTIR1
    iaaH
    iCasp9
    BlastR
    

    Use

    Synthetic Biology

    Tags

    mAID, mCherry, and mGFPmut3

    Expression

    Mammalian

    Mutation

    Promoter

    no promoter, T2A is used and pEF1s

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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